• PMID: 8258731
• Gene Name: EGF
• Condition: Stimulates mouse sperm capacitation
• Association: Associated
• Sex: Male
• Infertility type: Male infertility
• Associated genes: EGF
• Other associated phenotypes: Stimulates mouse sperm capacitation

[Effect of epidermal growth factor (EGF) on mouse sperm capacitation]

Furuya S, Endo Y, Oba M, Ito T, Suzuki S, Nozawa S.

The effect of epidermal growth factor (EGF) on the kinetics of mouse sperm capacitation was examined. The chlortetracycline (CTC) fluorescence assay was used to monitor capacitated sperm, featuring a B pattern. One hundred ng/ml EGF significantly stimulated capacitation at 15 minutes after the addition. However, 0.1 pg/ml EGF did not stimulate capacitation. EGF affected neither the motility nor the appearance of the S or AR pattern. The EGF-induced stimulation of capacitation was attenuated by 20 micrograms/ml genistein, an inhibitor of protein tyrosine kinase, or by 100 ng/ml thapsigargin, a non-phorbol tumor promoter, which inhibits EGF action by enhancing phosphorylation of the threonine 669 residue of the EGF-receptor. However, 3 ng/ml 12-O-tetradecanoly phorbol 13-acetate (TPA), a stimulator of protein kinase C, did not attenuate the appearance of the B pattern enhanced by 100 ng/ml EGF. These data suggest that EGF stimulates mouse sperm capacitation by activating the tyrosine kinase of the EGF-receptor which is regulated by multisite phosphorylation. FAU - Furuya, S AU - Furuya S AD - Department of Obstetrics and Gynecology, Nerima General Hospital, Tokyo. FAU - Endo, Y AU - Endo Y FAU - Oba, M AU - Oba M FAU - Ito, T AU - Ito T FAU - Suzuki, S AU - Suzuki S