• PMID: 30550884
• Gene Name: ATP6V0A2
• Condition: Acrosomal de-acidification
• Association: Associated
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: Asthenozoospermia, Male infertility

Proteomic analyses reveal lower expression of TEX40 and ATP6V0A2 proteins related to calcium ion entry and acrosomal acidification in asthenozoospermic males

Sinha A, Singh V, Singh S, Yadav S.

AIMS: Idiopathic nature of male infertility disorder needs to be investigated by different horizons of molecular biology for its treatment and to device male contraceptive. Further, it can also aid in advancement of assisted reproductive technology (ART), as nowadays the failure and disquiets of ART are consistent. Herein, we have attempted to find out proteins responsible for male infertility by comparing proteome profile of sperms collected from normal control and asthenozoospermic (AS) males. MAIN METHODS: Differential proteome profiles were studied by 2-dimensional differential gel electrophoresis (2D-DIGE) and mass spectrometry. The confirmation of proteome profiling results was done by western blotting and ELISA. Quantitative reverse-transcription-PCR was also performed in an independent cohort of AS and normal individuals to investigate the transcriptional regulation of proteins. KEY FINDINGS: Although seven differentially regulated proteins were identified, highpoints of the study were two proteins, TEX40 and ATP6V0A2. Lower expression of a crucial sperm motility related protein, TEX40 is reported for the first time in clinically diagnosed AS males in the present investigation. Most likely with reference to previous findings the down regulation of TEX40 leads to fewer entries of calcium ions in the sperm and lower expression of ATP6V0A2 is responsible for acrosomal de-acidification. SIGNIFICANCE: Conclusively, the down regulation of these two proteins in AS males might result in diminished sperm motility. The findings can be worthwhile for male contraception and ART management besides their use for male infertility therapy. CI - Copyright © 2018 Elsevier Inc. All rights reserved. FAU - Sinha, Ashima AU - Sinha A AD - Department of Biophysics, All India Institute of Medical Sciences, New Delhi 110029, India; Department of Biochemistry, Sir Ganga Ram Hospital, New Delhi 110060, India. FAU - Singh, Virendra AU - Singh V AD - School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India. FAU - Singh, Sarman AU - Singh S AD - Department of Laboratory Medicine, Division of Clinical Microbiology & Molecular Medicine, All India Institute of Medical Sciences, New Delhi 110029, India; All India Institute of Medical Sciences, Bhopal, Madhya Pradesh 462020, India.