• PMID: 30229503
• Gene Name: HSPA5
• Condition: Asthenozoospermia
• Association: Associated
• Mutation: rs3216733, rs17840761, and rs17840762
• Population size: 800
• Population details: 800 (400 subjects with AZS patients, 400 healthy controls)
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: Asthenozoospermia

Association of GRP78 promoter polymorphisms and serum GRP78 level with risk of asthenozoospermia

Qin H, Wang R, Pang X, Wei Y, Yang F, Wang J.

PURPOSE: The aim of this study was undertaken to investigate the association of 78-kDa glucose-regulated protein (GRP78) gene promoter polymorphisms with risk of asthenozoospermia (AZS) men. In addition, we performed association analysis between GRP78 promoter mutations and serum GRP78 level in asthenozoospermia. METHODS: The study population comprised 400 subjects with AZS patients and 400 healthy controls. We assessed GRP78 rs3216733, rs17840761, and rs17840762 polymorphisms by using Snapshot SNP genotyping assays; serum GRP78 level was measured by enzyme-linked immunosorbent assay (ELISA). Semen quality was assessed by computer-assisted semen analysis. RESULTS: We found that rs3216733 was associated with increased risk of AZS (Gd vs. dd: adjusted OR = 1.42, 95% CI, 1.06-1.93, P = 0.020; Gd/GG vs. dd: adjusted OR = 1.43, 95% CI, 1.08-1.91, P = 0.013; G vs. d adjusted OR = 1.26, 95% CI, 1.03-1.56, P = 0.027). The haplotype analyses showed the frequency of G-C-C haplotype was significantly higher in AZS (P = 0.026). The percentage of progressive motility sperm was lower in the asthenozoospermic men with Gd and Gd/GG genotypes than dd genotype (P = 0.003). Moreover, the serum GRP78 levels were significantly lower in rs3216733 Gd/GG genotypes compared with the dd genotype (P < 0.001). CONCLUSION: Our findings suggest that rs3216733 Gd/GG genotypes contribute to poor sperm motility, probably by decreasing the level of GRP78. FAU - Qin, Haimei AU - Qin H AD - Department of Reproduction and Genetics, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China. AD - Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China. FAU - Wang, Rong AU - Wang R AD - Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China. AD - Department of Laboratory Medicine, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China. FAU - Pang, Xiaoxia AU - Pang X AD - Department of Reproduction and Genetics, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China. FAU - Wei, Yuxiao AU - Wei Y AD - Department of Reproduction and Genetics, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China. FAU - Yang, Fenglian AU - Yang F AD - Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China.