• PMID: 29892896
• Gene Name: DICER1
• Condition: Azoospermia
• Association: Associated
• Mutation: DICER1 (rs12323635, rs1057035, rs13078 and rs3742330), DROSHA (rs10719, rs642321 and rs2291102)
• Population size: 505
• Population details: 505 (385 infertile men, 120 fertile controls)
• Sex: Male
• Infertility type: Male infertility
• Associated genes: DROSHA
• Other associated phenotypes: Azoospermia

Association of rs1057035polymorphism in microRNA biogenesis pathway gene (DICER1) with azoospermia among Iranian population

Moghbelinejad S, Najafipour R, Momeni A.

Since genes involved in microRNA biogenesis pathways have a main role in impaired spermatogenesis, in this research, we evaluated different genotypes frequency of seven single-nucleotide polymorphisms in DICER1 and DROSHA genes. Different genotypes frequency of DICER1 (rs12323635, rs1057035, rs13078 and rs3742330) and DROSHA (rs10719, rs642321 and rs2291102) were determined by sequencing method in 385 infertile men and 120 fertile controls. It was found that CC genotype (P = 0.000) and C allele (P = 0.0) of rs1057035 T > C polymorphism were associated with idiopathic male infertility (azoospermia). Gene expression study in blood and testis samples was done by real time PCR technique. Our results showed significant under expression of DICER1 gene in blood and testis tissues of azoospermic samples (P < 0.05), but we did not observed significant difference in expression ratio between infertile men with and without C allele of rs1057035 SNP (P > 0.05). The results of this study showed that among the studied variants, only one of them in DICER1 might be associated with azoospermia, but additional studies needs in different populations and ethnics. FAU - Moghbelinejad, Sahar AU - Moghbelinejad S AD - Cellular and Molecular Research Centre, Qazvin University of Medical Sciences, P. O. Box 341197-5981, Qazvin, Iran. smoghbelinejad@qums.ac.ir. FAU - Najafipour, Reza AU - Najafipour R AD - Cellular and Molecular Research Centre, Qazvin University of Medical Sciences, P. O. Box 341197-5981, Qazvin, Iran.