• PMID: 29726644
• Gene Name: SLC22A14
• Condition: Idiopathic asthenozoospermia, Male infertility
• Association: Associated
• Population size: 100
• Population details: 100 (50 idiopathic asthenozoospermia patients, 50 normal sperm donors)
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: Idiopathic asthenozoospermia, Male infertility

[Expressions of SLC22A14 and SPAG6 proteins in the ejaculated sperm of idiopathic asthenozoospermia patients.]

Huo FY, Li YS, Yang XY, Wang QX, Liu JJ, Wang LK, Su YH, Sun L.

OBJECTIVE: To investigate the expressions of solute carrier family 22 member 14 (SLC22A14) and sperm-associated antigen 6 (SPAG6) in the sperm of idiopathic asthenospermia men. METHODS: We collected semen samples from 50 idiopathic asthenozoospermia patients and another 50 normal sperm donors, purified the sperm by discontinuous density centrifugation on Percoll gradients, and then determined the mRNA and protein expressions of SLC22A14 and SPAG6 by RT-PCR and Western blot, respectively. RESULTS: Compared with the normal controls, the idiopathic asthenozoospermia patients showed significantly decreased mRNA expressions of SLC22A14 (0.77 ± 0.08 vs 0.53 ± 0.10, P<0.01) and SPAG6 (0.78 ± 0.09 vs0.52 ± 0.10 , P<0.01) and protein expressions of SLC22A14 (0.80 ± 0.09 vs 0.55 ± 0.10 , P<0.01) and SPAG6 (0.78 ± 0.09 vs 0.56 ± 0.09, P<0.01). CONCLUSIONS: T The expressions of SLC22A14 and SPAG6 are reduced in the sperm of the patients with idiopathic asthenospermia, which may be one of the important causes of asthenospermia. FAU - Huo, Fang-Yuan AU - Huo FY AD - Human Sperm Bank, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China. FAU - Li, Yu-Shan AU - Li YS AD - Human Sperm Bank, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China. FAU - Yang, Xi-Yang AU - Yang XY AD - Human Sperm Bank, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China. FAU - Wang, Quan-Xian AU - Wang QX AD - Human Sperm Bank, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China. FAU - Liu, Jun-Jie AU - Liu JJ AD - Human Sperm Bank, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China. FAU - Wang, Lin-Kai AU - Wang LK AD - Human Sperm Bank, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China. FAU - Su, Yan-Hua AU - Su YH AD - Human Sperm Bank, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.