• PMID: 29658246
• Gene Name: DAXX
• Condition: Spermatogenesis defects, Male infertility
• Association: Associated
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: Spermatogenesis defects, Male infertility

[Expression characteristics of the Daxx gene in the mouse testis during spermatogenesis]

Zhang Z, Deng Q, Wu Y, Huang XB, Yao L, Jiang ZM, Gui YT.

OBJECTIVE: To investigate the expression characteristic of the Daxx gene in the mouse testis and its role in spermatogenesis. METHODS: Real-time PCR, Western blot and immunofluorescence were used in examining the expression characteristics of DAXX in the testis tissue from wild-type, Sertoli cell-specific androgen receptor knockout (SCARKO) and androgen receptor knockout (ARKO) mice at different postnatal weeks . RESULTS: The Daxx gene was highly expressed in the testis tissue and mainly in the nuclei of the wild-type mice at 4 postnatal weeks. Compared with the wild-type, the ARKO mice showed a markedly decreased expression of DAXX (0.299±0.026), which displayed a polar distribution in the spermatogenic cells (0.853±0.058) and exhibited no significant difference in the SCARKO mice (1.000±0.015). CONCLUSIONS: The Daxx gene expression is the highest in the middle-stage development of the mouse testis, significantly decreased in ARKO mice as compared with the wild-type, and its location influenced by specific AR knockout in Sertoli cells. DAXX may be involved in the regulation of spermatogenesis in mice. FAU - Zhang, Zeng AU - Zhang Z AD - Graduate School, Anhui Medical University, Hefei, Anhui 230032, China. FAU - Deng, Qiong AU - Deng Q AD - Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, China. FAU - Wu, Yong AU - Wu Y AD - Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, China. FAU - Huang, Xin-Bo AU - Huang XB AD - Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, China. FAU - Yao, Lu AU - Yao L AD - Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, China. FAU - Jiang, Zhi-Mao AU - Jiang ZM AD - Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, China.