• PMID: 29643039
• Gene Name: DKKL1
• Condition: Asthenospermia, Male infertility
• Association: Associated
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: Asthenospermia, Male infertility

[Expression of DKKL1 in spermatozoa of men with asthenospermia]

Yan QX, Ma Y, Chen RQ, Zhou XQ, Qiao J, Xian YJ, Feng L, Chen CR.

OBJECTIVE: To compare the expression of DKKL1 in ejaculated spermatozoa of normal fertile men and men with asthenospermia and investigate the role of DKKL1 in the pathogenesis of asthenospermia. METHODS: The characteristics of semen samples collected from normal fertile men and men with asthenospermia were analyzed using computer-assisted sperm analysis according to WHO criteria. The ejaculated sperms were isolated by Percoll discontinuous density gradients to detect the expression of DKKL1 mRNA and protein using real-time PCR and Western blotting. RESULTS: The expression of DKKL1 mRNA was significantly down-regulated by 11.1 times in asthenospermic men as compared with that in normal fertile men (P<0.01). Western blotting showed that the expression of DKKL1 protein was down-regulated by 2.4 times in asthenospermic men compared to normal fertile men. CONCLUSION: The expression of DKKL1, which may play an important role in sperm motility,is significantly decreased in ejaculated spermatozoa of men with asthenospermia. FAU - Yan, Qiu-Xia AU - Yan QX AD - 1Center for Reproductive Medicine, Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People's Hospital, Qingyuan 511518,China; 2Department of Cellular Biology, Institute of Biological Medicine, Jinan University, Guangzhou 510632, China. E-mail: yanqiuxia1982@163.com. FAU - Ma, Yi AU - Ma Y FAU - Chen, Run-Qiang AU - Chen RQ FAU - Zhou, Xiu-Qin AU - Zhou XQ FAU - Qiao, Jing AU - Qiao J FAU - Xian, Ying-Jie AU - Xian YJ FAU - Feng, Ling AU - Feng L