• PMID: 29397567
• Gene Name: PLCG1
• Condition: Sperm-borne oocyte activating defects, fertilization failure, male infertility
• Association: Associated
• Population size: 30
• Population details: 30 (15 fertile men (control group) and 15 patients with previous fertilization failure following ICSI (FF group))
• Sex: Male
• Infertility type: Male infertility
• Associated genes: WBP2NL, PLCG1
• Other associated phenotypes: Sperm-borne oocyte activating defects, fertilization failure, male infertility

Evaluation of PAWP and PLC? Expression in Infertile Men with Previous ICSI Fertilization Failure

Azad N, Nazarian H, Nazari L, Ghaffari Novin M, Piryaei A, Heidari MH, Masteri Farahani R, Sadjadpour SS.

PURPOSE: The aim of this study was to evaluate postacrosomal sheet WW domain binding protein (PAWP) and phospholipase C ? (PLC?) protein expression in patients with fertilization failure. MATERIALS AND METHODS: Semen samples were collected from 15 fertile men (control group) and 15 patients with previous fertilization failure following ICSI (FF group) and were analyzed according to World Health Organization (WHO) criteria. The mean percentages of PAWP and PLC? positive sperm and the total level of PAWP and PLC? proteins were assessed using immunofluorescence staining. RESULTS: A significantly lower level and lower percentage of PAWP positive sperm in patients with fertilization failure was found compared to the control group (P = 0.01 and P = 0.03, respectively). The mean percentage ofPLC? positive sperm and level of PLC? protein were significantly lower in FF group compared to the control group (P = 0.0003 and P = 0.04, respectively). Significant positive correlations was observed between PAWP and PLC? positive sperms (r = 0.4, P = 0.008) and also total level of expression of PLC? and PAWP proteins (r = 0.4, P = 0.02) in all participants in the study. CONCLUSION: This is the first study that evaluates two main candidates for sperm-borne oocyte activating factors (SOAFs) simultaneously in patients with fertilization failure. Considering lower expression of PAWP and PLC? proteins in such patients, it seems like both factors might have the potential to be considered as SOAFs and diagnostic markers for the oocyte activation ability. FAU - Azad, Nahid AU - Azad N AD - Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. nazad1390@gmail.com. FAU - Nazarian, Hamid AU - Nazarian H AD - Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. FAU - Nazari, Leila AU - Nazari L AD - Department of Obstetrics and Gynecology, preventative Gynecology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. FAU - Ghaffari Novin, Marefat AU - Ghaffari Novin M AD - Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. FAU - Piryaei, Abbas AU - Piryaei A AD - Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. FAU - Heidari, Mohammad Hassan AU - Heidari MH AD - Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. FAU - Masteri Farahani, Reza AU - Masteri Farahani R AD - Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.