• PMID: 29293958
• Gene Name: CDC14A
• Condition: Male infertility
• Association: Associated
• Mutation: p.C278S
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: Male infertility

CDC14A phosphatase is essential for hearing and male fertility in mouse and human

Imtiaz A, Belyantseva IA, Beirl AJ, Fenollar-Ferrer C, Bashir R, Bukhari I, Bouzid A, Shaukat U, Azaiez H, Booth KT, Kahrizi K, Najmabadi H, Maqsood A, Wilson EA, Fitzgerald TS, Tlili A, Olszewski R, Lund M, Chaudhry T, Rehman AU, Starost MF, Waryah AM, Hoa M, Dong L, Morell RJ, Smith RJH, Riazuddin S, Masmoudi S, Kindt KS, Naz S, Friedman TB.

The Cell Division-Cycle-14 gene encodes a dual-specificity phosphatase necessary in yeast for exit from mitosis. Numerous disparate roles of vertebrate Cell Division-Cycle-14 (CDC14A) have been proposed largely based on studies of cultured cancer cells in vitro. The in vivo functions of vertebrate CDC14A are largely unknown. We generated and analyzed mutations of zebrafish and mouse CDC14A, developed a computational structural model of human CDC14A protein and report four novel truncating and three missense alleles of CDC14A in human families segregating progressive, moderate-to-profound deafness. In five of these families segregating pathogenic variants of CDC14A, deaf males are infertile, while deaf females are fertile. Several recessive mutations of mouse Cdc14a, including a CRISPR/Cas9-edited phosphatase-dead p.C278S substitution, result in substantial perinatal lethality, but survivors recapitulate the human phenotype of deafness and male infertility. CDC14A protein localizes to inner ear hair cell kinocilia, basal bodies and sound-transducing stereocilia. Auditory hair cells of postnatal Cdc14a mutants develop normally, but subsequently degenerate causing deafness. Kinocilia of germ-line mutants of mouse and zebrafish have normal lengths, which does not recapitulate the published cdc14aa knockdown morphant phenotype of short kinocilia. In mutant male mice, degeneration of seminiferous tubules and spermiation defects result in low sperm count, and abnormal sperm motility and morphology. These findings for the first time define a new monogenic syndrome of deafness and male infertility revealing an absolute requirement in vivo of vertebrate CDC14A phosphatase activity for hearing and male fertility. FAU - Imtiaz, Ayesha AU - Imtiaz A AD - Laboratory of Molecular Genetics, National Institute on Deafness and Other Communication Disorders, NIH, Bethesda, MD 20892, USA. AD - School of Biological Sciences, University of the Punjab, Lahore 54590, Pakistan. FAU - Belyantseva, Inna A AU - Belyantseva IA AD - Laboratory of Molecular Genetics, National Institute on Deafness and Other Communication Disorders, NIH, Bethesda, MD 20892, USA. FAU - Beirl, Alisha J AU - Beirl AJ AD - Section on Sensory Cell Development and Function, National Institute on Deafness and Other Communication Disorders, NIH, Bethesda, MD 20892, USA. FAU - Fenollar-Ferrer, Cristina AU - Fenollar-Ferrer C AD - Laboratory of Molecular and Cellular Neurobiology, Section on Molecular and Cellular Signaling, National Institute of Mental Health, NIH, Bethesda, MD 20892, USA. FAU - Bashir, Rasheeda AU - Bashir R AD - School of Biological Sciences, University of the Punjab, Lahore 54590, Pakistan. FAU - Bukhari, Ihtisham AU - Bukhari I AD - School of Biological Sciences, University of the Punjab, Lahore 54590, Pakistan. FAU - Bouzid, Amal AU - Bouzid A AD - Laboratoire Procédés de Criblage Moléculaire et Cellulaire, Centre de Biotechnologie de Sfax, Université de Sfax, Sfax 3451, Tunisia. FAU - Shaukat, Uzma AU - Shaukat U AD - Center of Excellence in Molecular Biology, University of the Punjab, Lahore 54590, Pakistan. FAU - Azaiez, Hela AU - Azaiez H AD - Molecular Otolaryngology and Renal Research Laboratories, Department of Otolaryngology - Head and Neck Surgery, University of Iowa, Iowa City, 52242, IA, USA. FAU - Booth, Kevin T AU - Booth KT AD - Molecular Otolaryngology and Renal Research Laboratories, Department of Otolaryngology - Head and Neck Surgery, University of Iowa, Iowa City, 52242, IA, USA. AD - The Interdisciplinary Graduate Program in Molecular Medicine, Carver College of Medicine, University of Iowa, Iowa City, 52242, IA, USA. FAU - Kahrizi, Kimia AU - Kahrizi K AD - Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran 1987513834, Iran. FAU - Najmabadi, Hossein AU - Najmabadi H AD - Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran 1987513834, Iran. FAU - Maqsood, Azra AU - Maqsood A AD - Laboratory of Molecular Genetics, National Institute on Deafness and Other Communication Disorders, NIH, Bethesda, MD 20892, USA. AD - School of Biological Sciences, University of the Punjab, Lahore 54590, Pakistan. FAU - Wilson, Elizabeth A AU - Wilson EA AD - Laboratory of Molecular Genetics, National Institute on Deafness and Other Communication Disorders, NIH, Bethesda, MD 20892, USA. FAU - Fitzgerald, Tracy S AU - Fitzgerald TS AD - Mouse Auditory Testing Core Facility, NIH, Bethesda, MD 20892, USA. FAU - Tlili, Abdelaziz AU - Tlili A AD - Laboratoire Procédés de Criblage Moléculaire et Cellulaire, Centre de Biotechnologie de Sfax, Université de Sfax, Sfax 3451, Tunisia. FAU - Olszewski, Rafal AU - Olszewski R AD - Auditory Development and Restoration Program, National Institute on Deafness and Other Communication Disorders, NIH, Bethesda, MD 20892, USA. FAU - Lund, Merete AU - Lund M AD - Laboratory of Molecular Genetics, National Institute on Deafness and Other Communication Disorders, NIH, Bethesda, MD 20892, USA. FAU - Chaudhry, Taimur AU - Chaudhry T AD - Laboratory of Molecular Genetics, National Institute on Deafness and Other Communication Disorders, NIH, Bethesda, MD 20892, USA. FAU - Rehman, Atteeq U AU - Rehman AU AD - Laboratory of Molecular Genetics, National Institute on Deafness and Other Communication Disorders, NIH, Bethesda, MD 20892, USA. FAU - Starost, Matthew F AU - Starost MF AD - Division of Veterinary Resources, National Institutes of Health, Bethesda, MD 20892, USA. FAU - Waryah, Ali M AU - Waryah AM AD - Center of Excellence in Molecular Biology, University of the Punjab, Lahore 54590, Pakistan. FAU - Hoa, Michael AU - Hoa M AD - Auditory Development and Restoration Program, National Institute on Deafness and Other Communication Disorders, NIH, Bethesda, MD 20892, USA. FAU - Dong, Lijin AU - Dong L AD - Genetic Engineering Core, National Eye Institute, NIH, Bethesda, MD 20892, USA. FAU - Morell, Robert J AU - Morell RJ AD - Genomics and Computational Biology Core, National Institute on Deafness and Other Communication Disorders, NIH, Bethesda, MD 20892, USA. FAU - Smith, Richard J H AU - Smith RJH AD - Molecular Otolaryngology and Renal Research Laboratories, Department of Otolaryngology - Head and Neck Surgery, University of Iowa, Iowa City, 52242, IA, USA. AD - The Interdisciplinary Graduate Program in Molecular Medicine, Carver College of Medicine, University of Iowa, Iowa City, 52242, IA, USA. FAU - Riazuddin, Sheikh AU - Riazuddin S AD - Center of Excellence in Molecular Biology, University of the Punjab, Lahore 54590, Pakistan. AD - Pakistan Institute of Medical Sciences, Shaheed Zulfiqar Ali Bhutto Medical University, Islamabad 44000, Pakistan. AD - Laboratory for Research in Genetic Diseases, Burn Centre, Allama Iqbal Medical College, University of Health Sciences, Lahore 54590, Pakistan. FAU - Masmoudi, Saber AU - Masmoudi S AD - Laboratoire Procédés de Criblage Moléculaire et Cellulaire, Centre de Biotechnologie de Sfax, Université de Sfax, Sfax 3451, Tunisia. FAU - Kindt, Katie S AU - Kindt KS AD - Section on Sensory Cell Development and Function, National Institute on Deafness and Other Communication Disorders, NIH, Bethesda, MD 20892, USA. FAU - Naz, Sadaf AU - Naz S AD - School of Biological Sciences, University of the Punjab, Lahore 54590, Pakistan.