• PMID: 29207172
• Gene Name: RANBP9
• Condition: Associated with human spermatogenesis
• Association: Associated
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: Associated with human spermatogenesis

Ran-binding protein M is associated with human spermatogenesis and oogenesis

Tang WH, Zhuang XJ, Song SD, Wu H, Zhang Z, Yang YZ, Zhang HL, Mao JM, Liu DF, Zhao LM, Lin HC, Hong K, Ma LL, Qiao J, Qin W, Tang Y, Jiang H.

The aim of the present study was to explore the underlying mechanism and diagnostic potential of Ran‑binding protein M (RanBPM) in human spermatogenesis and oogenesis. RanBPM expression in human testis and ovaries was analysed using polymerase chain reaction (PCR) and western blotting, and immunofluorescence was performed on testis and ovary tissue sections during different developmental stages of spermatogenesis and oogenesis using RanBPM antibodies. Interactions with a variety of functional proteins were also investigated. RanBPM mRNA and protein expression levels were determined by PCR and western blotting in the tissue sections. Results revealed that the mRNA expression levels were highest in the testis followed by the ovary. The RanBPM protein was predominantly localized in the nucleus of germ cells, and the expression levels were highest in pachytene spermatocytes and cells surrounding spermatids in testis tissue. In ovary cells, RanBPM was localized in the nucleus and cytoplasm. In conclusion, the results suggested that RanBPM may have multiple roles in the regulation of germ cell proliferation during human spermatogenesis and oogenesis. This research may provide a novel insight into the underlying molecular mechanism of RanBPM and may have implications for the clinical diagnosis and treatment of human infertility. FAU - Tang, Wen-Hao AU - Tang WH AD - 1Department of Urology, The Third Hospital of Peking University, Beijing 100191, P.R. China. FAU - Zhuang, Xin-Jie AU - Zhuang XJ AD - Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Ministry of Education, Peking University Third Hospital, Beijing 100191, P.R. China. FAU - Song, Shi-De AU - Song SD AD - Department of Urology, Rizhao People's Hospital, Rizhao, Shandong 276500, P.R. China. FAU - Wu, Han AU - Wu H AD - 1Department of Urology, The Third Hospital of Peking University, Beijing 100191, P.R. China. FAU - Zhang, Zhe AU - Zhang Z AD - 1Department of Urology, The Third Hospital of Peking University, Beijing 100191, P.R. China. FAU - Yang, Yu-Zhuo AU - Yang YZ AD - Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Ministry of Education, Peking University Third Hospital, Beijing 100191, P.R. China. FAU - Zhang, Hong-Liang AU - Zhang HL AD - Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Ministry of Education, Peking University Third Hospital, Beijing 100191, P.R. China. FAU - Mao, Jia-Ming AU - Mao JM AD - Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Ministry of Education, Peking University Third Hospital, Beijing 100191, P.R. China. FAU - Liu, De-Feng AU - Liu DF AD - Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Ministry of Education, Peking University Third Hospital, Beijing 100191, P.R. China. FAU - Zhao, Lian-Ming AU - Zhao LM AD - 1Department of Urology, The Third Hospital of Peking University, Beijing 100191, P.R. China. FAU - Lin, Hao-Cheng AU - Lin HC AD - 1Department of Urology, The Third Hospital of Peking University, Beijing 100191, P.R. China. FAU - Hong, Kai AU - Hong K AD - 1Department of Urology, The Third Hospital of Peking University, Beijing 100191, P.R. China. FAU - Ma, Lu-Lin AU - Ma LL AD - 1Department of Urology, The Third Hospital of Peking University, Beijing 100191, P.R. China. FAU - Qiao, Jie AU - Qiao J AD - Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Ministry of Education, Peking University Third Hospital, Beijing 100191, P.R. China. FAU - Qin, Weibing AU - Qin W AD - Key Laboratory of Male Reproduction and Genetics, National Health and Family Planning Commission, Family Planning Research Institute of Guangdong Province, Guangzhou, Guangdong 510600, P.R. China. FAU - Tang, Yunge AU - Tang Y AD - Key Laboratory of Male Reproduction and Genetics, National Health and Family Planning Commission, Family Planning Research Institute of Guangdong Province, Guangzhou, Guangdong 510600, P.R. China.