• PMID: 27802166
• Gene Name: CABYR
• Condition: Formation of fibrous sheath, Male infertility
• Association: Associated
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: Formation of fibrous sheath, Male infertility

CABYR is essential for fibrous sheath integrity and progressive motility in mouse spermatozoa

Young SA, Miyata H, Satouh Y, Aitken RJ, Baker MA, Ikawa M.

Ca(2+)-binding tyrosine-phosphorylation-regulated protein (CABYR) has been implicated in sperm physiological function in several in vitro studies. It has also been implicated as a potential cause of and diagnostic tool in asthenozoospermic human males. CABYR is known to be localized to the fibrous sheath, an accessory structure in the flagellar principal piece. Utilizing the CRISPR-Cas9 technology, we have knocked out this gene in mice to understand its role in male fertility. Cabyr-knockout male mice showed severe subfertility with a defect in sperm motility as well as a significant disorganization in the fibrous sheath. Further, abnormal configuration of doublet microtubules was observed in the Cabyr-knockout spermatozoa, suggesting that the fibrous sheath is important for the correct organization of the axoneme. Our results show that it is the role of CABYR in the formation of the fibrous sheath that is essential for male fertility. CI - © 2016. Published by The Company of Biologists Ltd. FAU - Young, Samantha A M AU - Young SA AD - Priority Research Centre in Reproductive Science, Discipline of Biological Sciences, Faculty of Science and IT, University of Newcastle, Callaghan, New South Wales 2308, Australia. AD - Department of Experimental Genome Research, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan. FAU - Miyata, Haruhiko AU - Miyata H AD - Department of Experimental Genome Research, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan. FAU - Satouh, Yuhkoh AU - Satouh Y AD - Animal Resource Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan. FAU - Aitken, Robert John AU - Aitken RJ AD - Priority Research Centre in Reproductive Science, Discipline of Biological Sciences, Faculty of Science and IT, University of Newcastle, Callaghan, New South Wales 2308, Australia. FAU - Baker, Mark A AU - Baker MA AD - Priority Research Centre in Reproductive Science, Discipline of Biological Sciences, Faculty of Science and IT, University of Newcastle, Callaghan, New South Wales 2308, Australia.