• PMID: 27760631
• Gene Name: INHBB
• Condition: Spermatogenesis dysfunction, Male infertility
• Association: Associated
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: Spermatogenesis dysfunction, Male infertility

[Relationship between decreased expression of inhibin B and spermatogenesis dysfunction in rats testis]

Zhang J, Jin PP, Fang K, Yi QT, Tang MF, Chen CH, Guo JH, Gong M.

Objective: To explore the effects of varicocele on the expressions of inhibin B in rat testes and the relationship between expression of inhibin B and spermatogenesis dysfunction. Methods: Twenty specific-pathogen-free Sprague-Dawley (SD) male rats were randomly divided into two groups with random number table: 4-week control group (C4) and 4-week experimental group(V4). Experimental varicocele was created by partial ligation of left renal vein in the V4 group; vein isolation without ligation was performed in the C4 group.Spermatogenetic function in the two groups were assessed. The expression of inhibin B in the rat testis was analyzed by immunohistochemistry, the expressions of inhibin B, Fas and Fas ligand(FasL)mRNA measured by reverse transcription-polymerase chain reaction(RT-PCR), and the inhibin B protein expression by Western blot. Results: In comparison of spermatogenetic function in left rat testes, the Johnsen scores in the V4 group were significantly lower than those in the C4 group (9.79±0.05 vs 9.97±0.02, P=0.023), the seminiferous epithelium in the V4 group was significantly thinner than that in the C4 group [(48.35±0.99)μm vs (57.58±1.98)μm, P=0.000], and the number of sperms in the left epididymis was significantly lower than in the right one in the V4 group [(933±161)×10(6)/(ml·g) vs(1 552±184)×10(6)/(ml·g), P=0.017]. Both Western blot and immunohistochemical assay showed that the expression of inhibin B in the rat testes was significantly lower in the V4 group than in the C4 group(0.407±0.053 vs 0.608±0.076, P=0.038; 0.161±0.004 vs 0.183±0.005, P=0.008). RT-PCR also detected reduced expression of inhibin B mRNA in the V4 group compared with the C4 group(0.522±0.050 vs 1.106±0.210, P=0.003. Compared to the C4 group, the expression of Fas mRNA in the V4 group was significantly lower, while the expression of FasL mRNA in the V4 group was significantly higher(P=0.019, 0.015). Conclusions: Varicocele can lead to decreased expressions of inhibin B in rat testes and cause spermatogenesis dysfunction. There may be a close correlation between down-regulation ofinhibin B expression and spermatogenesis dysfunction.Inhibin B may play a significant rolein the mechanisms underlying male infertility due to varicocele. FAU - Zhang, J AU - Zhang J AD - Department of Urology, Shanghai Pudong Hospital, Fudan University, Shanghai 201399, China. FAU - Jin, P P AU - Jin PP AD - Department of Urology, Shanghai Pudong Hospital, Fudan University, Shanghai 201399, China. FAU - Fang, K AU - Fang K AD - Department of Urology, Shanghai Pudong Hospital, Fudan University, Shanghai 201399, China. FAU - Yi, Q T AU - Yi QT AD - Department of Urology, Shanghai Pudong Hospital, Fudan University, Shanghai 201399, China. FAU - Tang, M F AU - Tang MF AD - Department of Urology, Shanghai Pudong Hospital, Fudan University, Shanghai 201399, China. FAU - Chen, C H AU - Chen CH AD - Department of Urology, Shanghai Pudong Hospital, Fudan University, Shanghai 201399, China. FAU - Guo, J H AU - Guo JH AD - Department of Urology, Shanghai Pudong Hospital, Fudan University, Shanghai 201399, China.