• PMID: 27635527
• Gene Name: CDC42
• Condition: Prevents actin polymerization during capacitation, Male infertility
• Association: Associated
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: Prevents actin polymerization during capacitation, Male infertility

Absence of aryl hydrocarbon receptor alters CDC42 expression and prevents actin polymerization during capacitation

Angeles-Floriano T, Roa-Espitia AL, Baltiérrez-Hoyos R, Cordero-Martínez J, Elizondo G, Hernández-González EO.

The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor that regulates the toxicity of a variety of environmental chemicals. The absence of this receptor causes serious reproductive complications. Ahr-knockout (Ahr-KO) male mice, for example, are considerably less fertile: Half of the few spermatozoa they produce exhibit morphological alterations, and those with typical morphology may have pathologic modifications. We therefore investigated the consequences of AHR loss on capacitation and the acrosome reaction, and asked if these effects are a consequence of changes to actin polymerization and the expression of Cdc42, which encodes Cell division control protein 42 (CDC42), a RHO protein that controls assembly of the actin cytoskeleton in somatic cells as well as during spermatogenesis. Nearly 50% of spermatozoa produced by Ahr-KO mice had alterations in the flagellum. Ahr-KO spermatozoa were frequently capacitated, but showed reduced spontaneous and progesterone-induced acrosome reaction-which is related to low CDC42 abundance and very limited actin polymerization during capacitation. Thus, the expression of CDC42 might be regulated by AHR, and both proteins are fundamental to the development of normal spermatozoa and the acrosome reaction. Mol. Reprod. Dev. 83: 1015-1026, 2016 © 2016 Wiley Periodicals, Inc. CI - © 2016 Wiley Periodicals, Inc. FAU - Angeles-Floriano, Tania AU - Angeles-Floriano T AD - Departamento de Biología Celular, CINVESTAV-IPN, Av. Instituto Politécnico Nacional 2508, CP 07360, México DF, México. FAU - Roa-Espitia, Ana L AU - Roa-Espitia AL AD - Departamento de Biología Celular, CINVESTAV-IPN, Av. Instituto Politécnico Nacional 2508, CP 07360, México DF, México. FAU - Baltiérrez-Hoyos, Rafael AU - Baltiérrez-Hoyos R AD - Facultad de Medicina y Cirugía, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca, Cátedras CONACYT. FAU - Cordero-Martínez, Joaquin AU - Cordero-Martínez J AD - Departamento de Biología Celular, CINVESTAV-IPN, Av. Instituto Politécnico Nacional 2508, CP 07360, México DF, México. FAU - Elizondo, Guillermo AU - Elizondo G AD - Departamento de Biología Celular, CINVESTAV-IPN, Av. Instituto Politécnico Nacional 2508, CP 07360, México DF, México.