• PMID: 27612028
• Gene Name: TRIM27
• Condition: Influences germ cell proliferation in spermatogenesis, Male infertility
• Association: Associated
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: Influences germ cell proliferation in spermatogenesis, Male infertility

Trim27 interacts with Slx2, is associated with meiotic processes during spermatogenesis

Zhuang XJ, Tang WH, Feng X, Liu CY, Zhu JL, Yan J, Liu DF, Liu P, Qiao J.

ABSTARCT Formation of the XY body is believed to prevent recombination between X and Y chromosomes during meiosis. We recently demonstrated that SYCP3-like X-linked 2 (Slx2) could be involved in synaptonemal complex formation as well as XY body maintenance during meiosis. In order to further investigate the role and composition of XY body protein complexes in meiotic processes and spermatogenesis, a yeast 2-hybrid screening was performed, and the tripartite motif protein 27(Trim27) was found to interact with Slx2 and co-localized in the XY body. Trim27 has a tripartite motif (TRIM) consisting of a RING finger, B-box and coiled-coil domains, and is a transcriptional regulator that is expressed in various tumor cell lines. In this study, we showed that Slx2 and Trim27 were highly expressed in meiosis of mouse testis. And the Slx2/Trim27 interaction was confirmed in vivo by co-immunoprecipitation and mammalian 2-hybrid interaction assays. Moreover, cytoimmuno localization experiments revealed that Slx2/Trim27 was co-localized to the XY body of spermatocytes during meiosis, and immunohistochemical results revealed co-localization of Trim27 and γ-H2AX in the XY body of primary spermatocytes in the mouse testis. Trim27 may therefore be a transcriptional regulation protein connecting Slx2 and γ-H2AX, thereby promoting the formation of a more potent XY body protein complex in meiotic processes and spermatogenesis. In conclusion, Trim27 connecting Slx2 may regulate meiotic processes in multiple ways by influencing XY body formation and germ cell proliferation during spermatogenesis. FAU - Zhuang, Xin-Jie AU - Zhuang XJ AD - a Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Ministry of Education, Peking University Third Hospital , Beijing , PR China. FAU - Tang, Wen-Hao AU - Tang WH AD - b Department of Urology , the Third Hospital of Peking University , Beijing , PR China. FAU - Feng, Xue AU - Feng X AD - a Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Ministry of Education, Peking University Third Hospital , Beijing , PR China. FAU - Liu, Chang-Yu AU - Liu CY AD - a Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Ministry of Education, Peking University Third Hospital , Beijing , PR China. FAU - Zhu, Jin-Liang AU - Zhu JL AD - a Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Ministry of Education, Peking University Third Hospital , Beijing , PR China. FAU - Yan, Jie AU - Yan J AD - a Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Ministry of Education, Peking University Third Hospital , Beijing , PR China. FAU - Liu, De-Feng AU - Liu DF AD - b Department of Urology , the Third Hospital of Peking University , Beijing , PR China. FAU - Liu, Ping AU - Liu P AD - a Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Ministry of Education, Peking University Third Hospital , Beijing , PR China.