• PMID: 27280388
• Gene Name: MAPKAPK2
• Condition: Regulates spermatogenesis, Male infertililty
• Association: Associated
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: Regulates spermatogenesis, Male infertililty

Phosphorylation of the RNA-binding protein Dazl by MAPKAP kinase 2 regulates spermatogenesis

Williams PA, Krug MS, McMillan EA, Peake JD, Davis TL, Cocklin S, Strochlic TI.

Developing male germ cells are exquisitely sensitive to environmental insults such as heat and oxidative stress. An additional characteristic of these cells is their unique dependence on RNA-binding proteins for regulating posttranscriptional gene expression and translational control. Here we provide a mechanistic link unifying these two features. We show that the germ cell-specific RNA-binding protein deleted in azoospermia-like (Dazl) is phosphorylated by MAPKAP kinase 2 (MK2), a stress-induced protein kinase activated downstream of p38 MAPK. We demonstrate that phosphorylation of Dazl by MK2 on an evolutionarily conserved serine residue inhibits its interaction with poly(A)-binding protein, resulting in reduced translation of Dazl-regulated target RNAs. We further show that transgenic expression of wild-type human Dazl but not a phosphomimetic form in the Drosophila male germline can restore fertility to flies deficient in boule, the Drosophila orthologue of human Dazl. These results illuminate a novel role for MK2 in spermatogenesis, expand the repertoire of RNA-binding proteins phosphorylated by this kinase, and suggest that signaling by the p38-MK2 pathway is a negative regulator of spermatogenesis via phosphorylation of Dazl. CI - © 2016 Williams et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). FAU - Williams, Patrick A AU - Williams PA AD - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102. FAU - Krug, Michael S AU - Krug MS AD - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102. FAU - McMillan, Emily A AU - McMillan EA AD - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102. FAU - Peake, Jasmine D AU - Peake JD AD - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102. FAU - Davis, Tara L AU - Davis TL AD - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102. FAU - Cocklin, Simon AU - Cocklin S AD - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102.