• PMID: 27264812
• Gene Name: miR-141-5p
• Condition: Idiopathic asthenospermia
• Association: Associated
• Sex: Male
• Infertility type: Male infertility
• Associated genes: miR-122-3p, miR-141-5p
• Other associated phenotypes: Idiopathic asthenospermia

[Seminal plasma miR-122-3p and miR-141-5p stability and its diagnosis value for idiopathic asthenospermia]

Zhang X, Wei R, Lou J, Zhou J.

OBJECTIVE: To explore the stability of seminal plasma miR-122-3p and miR-141-5p and their diagnostic value for idiopathic asthenospermia. METHODS: Seminal plasma miR-122-3p and miR-141-5p with various incubation time, freeze-thaw cycles and incubation time were analyzed. Real-time fluorescence quantitative polymerase chain reaction (RT-PCR) was used to detect the content of miR-122-3p and miR-141-5p, with U6 snRNA as the reference. The idiopathic asthenospermia group was further divided into grade I and II based on the progressive motility percent, and the content of miR-122-3p and miR-141-5p in the two grades were compared. RESULTS: No difference was detected after incubation at room temperature and 4 degrees Celsius. But seminal plasma miR-122-3p and miR-141-5p have decreased along with the increase in freeze-thaw cycles increases. RT-PCR assayed showed that the miR-122-3p content in the idiopathic asthenospermia group was significantly lower than the control group, while miR-141-5p was significantly higher. The difference in both miR-122-3p and miR-141-5p content was statistically significant (P < 0.05). A significant difference in miR-122-3p and miR-141-5p content between a and b groups was also detected (P < 0.05). The AUC-ROC of miR-122-3p was 0.88. At the cutoff value of 1.02, the sensitivity and specificity was 83% and 84%, respectively. At the cutoff of 2.95, the AUC-ROC of miR-141-5p was 0.88. The sensitivity and specificity was 84% and 70%, respectively. CONCLUSION: MiR-122-3p and miR-141-5p in seminal plasma are stable and have certain value for the diagnosis of idiopathic asthenospermia. FAU - Zhang, Xiaoxia AU - Zhang X AD - Ningbo Hospital of Traditional Chinese Medicine Affiliated to Zhejiang University of Traditional Chinese Medicine, Ningbo, Zhejiang 315012, China. nbxiaowei@163.com. FAU - Wei, Renxiong AU - Wei R FAU - Lou, Jiangtao AU - Lou J