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PMID 26832966
Gene Name DEFB126
Condition Male subfertility
Association Associated
Mutation del/del


Lectin binding of human sperm associates with DEFB126 mutation and serves as a potential biomarker for subfertility

Xin A, Cheng L, Diao H, Wu Y, Zhou S, Shi C, Sun Y, Wang P, Duan S, Zheng J, Wu B, Yuan Y, Gu Y, Chen G, Sun X, Shi H, Tao S, Zhang Y.

Coating on the sperm surface, glycocalyx, plays a key role in sperm motility, maturation and fertilization. A comprehensive profile of sperm surface glycans will greatly facilitate both basic researches and clinical studies. Because of the capability of recognizing different glycan moieties, lectins are widely used in glycobiology. However, lacking high-throughput technology, limited lectins have been reported for analyzing the glycan of human sperm. In this study, we employed a lectin microarray for profiling the surface glycans of human sperm, on which 54 out of 91 lectins showed positive binding. Based on this technique, we compared lectin binding profiling of sperm with homozygous DEFB126 mutation (del/del) with that of wild type (wt/wt). DEFB126 was reported to contribute to the sialylation on sperm surface and its homozygous mutation was related to male subfertility. Six lectins (Jacalin/AIA, GHA, ACL, MPL, VVL and ABA) were found to develop lower binding affinity to sperm with del/del. Further validation showed that these lectins, especially ABA and MPL, can be potential biomarkers for clinical diagnosis of subfertility due to the mutation of DEFB126. Our research provides insight into the detection of some unexplained male subfertility, and the lectin microarray is generally applicable for infertility/subfertility sperm biomarker discovery. FAU - Xin, Aijie AU - Xin A AD - Shanghai Ji Ai Genetics and IVF Institute, Obstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai 200011, China. FAU - Cheng, Li AU - Cheng L AD - Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China. FAU - Diao, Hua AU - Diao H AD - China National Population and Family Planning Key Laboratory of Contraceptive Drugs and Devices, SIPPR, Shanghai 200032, China. FAU - Wu, Yancheng AU - Wu Y AD - China National Population and Family Planning Key Laboratory of Contraceptive Drugs and Devices, SIPPR, Shanghai 200032, China. FAU - Zhou, Shumin AU - Zhou S AD - Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China. FAU - Shi, Changgen AU - Shi C AD - China National Population and Family Planning Key Laboratory of Contraceptive Drugs and Devices, SIPPR, Shanghai 200032, China. FAU - Sun, Yangyang AU - Sun Y AD - Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China. FAU - Wang, Peng AU - Wang P AD - China National Population and Family Planning Key Laboratory of Contraceptive Drugs and Devices, SIPPR, Shanghai 200032, China. FAU - Duan, Shiwei AU - Duan S AD - The Affiliated Hospital, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, China. FAU - Zheng, Jufen AU - Zheng J AD - China National Population and Family Planning Key Laboratory of Contraceptive Drugs and Devices, SIPPR, Shanghai 200032, China. FAU - Wu, Bin AU - Wu B AD - China National Population and Family Planning Key Laboratory of Contraceptive Drugs and Devices, SIPPR, Shanghai 200032, China. FAU - Yuan, Yao AU - Yuan Y AD - China National Population and Family Planning Key Laboratory of Contraceptive Drugs and Devices, SIPPR, Shanghai 200032, China. FAU - Gu, Yihua AU - Gu Y AD - China National Population and Family Planning Key Laboratory of Contraceptive Drugs and Devices, SIPPR, Shanghai 200032, China. FAU - Chen, Guowu AU - Chen G AD - Shanghai Ji Ai Genetics and IVF Institute, Obstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai 200011, China. FAU - Sun, Xiaoxi AU - Sun X AD - Shanghai Ji Ai Genetics and IVF Institute, Obstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai 200011, China. FAU - Shi, Huijuan AU - Shi H AD - Shanghai Ji Ai Genetics and IVF Institute, Obstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai 200011, China. AD - China National Population and Family Planning Key Laboratory of Contraceptive Drugs and Devices, SIPPR, Shanghai 200032, China. AD - Shanghai Key Laboratory of Reproductive Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. FAU - Tao, Shengce AU - Tao S AD - Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China.