• PMID: 26679162
• Gene Name: KIR2DS2
• Condition: Cryptorchidism
• Association: No association between KIR ligands C1 and C2, alone or together with KIR2DL2, was found. However, the results suggest that KIR2DL2+/KIR2DS2+ genotype may be, to some extent, protective againstcryptorchidism.
• Population size: 245
• Population details: 245 (109 prepubertal boys with cryptorchidism, 136 ethnically matched young male donors)
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: Cryptorchidism

Killer cell immunoglobulin-like receptor gene association with cryptorchidism

Niepiek?o-Miniewska W, Ku?nierczyk P, Havrylyuk A, Kamieniczna M, Nakonechnyy A, Chopyak V, Kurpisz M.

Cryptorchidism is a condition where a testis persists in the abdominal cavity. Thus, due to elevated temperature we may expect induction of aberrant immune reactions depending on genetic constitution of individual. This may be reflected by development of anti-sperm antibodies (ASA) in cryptorchid males. Also, natural killer (NK) cells which belong to innate immunity may control adaptive immunity. Therefore, the gene system encoding polymorphic NK cell immunoglobulin receptors (KIRs) has been studied. 109 prepubertal boys with cryptorchidism and 136 ethnically matched young male donors were selected to study NK cell KIRs. DNA was isolated using automatic Maxwell(®) system from the peripheral venous blood drawn onto anticoagulant. Olerup SSP KIR Genotyping kit including Taq polymerase was used for detection of KIR genes. Human leukocyte antigen-C (HLA-C) groups, C1 and C2 were established using a Olerup SSP KIR HLA Ligand kit. KIR2DL2 (killer immunoglobulin-like receptor two-domain long 2) and KIR2DS2 (killer immunoglobulin-like receptor two-domain short 2) genes were less frequent in patients than in control individuals (corrected p values: 0.0110 and 0.0383, respectively). However, no significant differences were observed between ASA-positive and ASA-negative patients, or between bilateral or unilateral cryptorchidism. No association between KIR ligands C1 and C2, alone or together with KIR2DL2, was found. However, the results suggest that KIR2DL2+/KIR2DS2+ genotype may be, to some extent, protective against cryptorchidism. CI - Copyright © 2015 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved. FAU - Niepiekło-Miniewska, Wanda AU - Niepiekło-Miniewska W AD - Laboratory of Immunogenetics and Tissue Immunology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland. Electronic address: niepie@iitd.pan.wroc.pl. FAU - Kuśnierczyk, Piotr AU - Kuśnierczyk P AD - Laboratory of Immunogenetics and Tissue Immunology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland. Electronic address: pkusnier@iitd.pan.wroc.pl. FAU - Havrylyuk, Anna AU - Havrylyuk A AD - Danylo Halytsky Lviv National Medical University, Department of Clinical Immunology and Allergology, Lviv, Ukraine. Electronic address: ahavrylyuk@meta.ua. FAU - Kamieniczna, Marzena AU - Kamieniczna M AD - Department of Reproductive Biology and Stem Cells, Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland. Electronic address: kaspmarz@man.poznan.pl. FAU - Nakonechnyy, Andrij AU - Nakonechnyy A AD - Danylo Halytsky Lviv National Medical University, Department of Pediatric Surgery, Lviv, Ukraine. Electronic address: andrurol@gmail.com. FAU - Chopyak, Valentyna AU - Chopyak V AD - Danylo Halytsky Lviv National Medical University, Department of Clinical Immunology and Allergology, Lviv, Ukraine. Electronic address: chopyakv@ukr.net.