• PMID: 26568756
• Gene Name: SOD
• Condition: Male infertility
• Association: These results suggested that oxidative stress induced sperm DNA damage might have a critical effect on the etiology of infertility. Therefore, evaluation of oxidative status, antioxidant defense systems and DNA damage, together with sperm parameters might
• Population size: 66
• Population details: 66 (25 fertile men, 41 infertile men)
• Sex: Male
• Infertility type: Male infertility
• Associated genes: SOD, MDA, PHP and 8-OHdG
• Other associated phenotypes: Male infertility

Oxidative stress induced sperm DNA damage, a possible reason for male infertility

Hosen MB, Islam MR, Begum F, Kabir Y, Howlader MZ.

BACKGROUND: Sperm DNA damage is an important factor in the etiology of male infertility. OBJECTIVE: The aim of the study was to evaluate the association of oxidative stress induced sperm DNA damage with the pathogenesis of male infertility. MATERIALS AND METHODS: The study comprised a total of 66 subjects, including fertile men (n=25) and infertile men (n=41) matched by age. Seminal malondialdehyde (MDA), phospholipid hydroperoxide (PHP), superoxide dismutase (SOD), total antioxidant status (TAS) and 8-hydroxy-2'-deoxy guanosine (8-OHdG) were estimated by spectrophotometric and ELISA based methods and the association with the sperm parameters was assessed. RESULTS: The percentages of motile and morphologically normal cells were significantly lower (p < 0.001, p <0.001, respectivly) in infertile men. Seminal levels of MDA, PHP and 8-OHdG were significantly higher (p < 0.001, p < 0.001, and p=0. 02, respectively) while the SOD and TAS were significantly lower (p=0. 0003, p< 0.001, respectively) in infertile men. Sperm parameters were negatively correlated with MDA, PHP and 8-OHdG while positively correlated with SOD and TAS. A positive correlation of 8-OHdG with MDA and PHP and a negative correlation with TAS and SOD were also found. CONCLUSION: These results suggested that oxidative stress induced sperm DNA damage might have a critical effect on the etiology of infertility. Therefore, evaluation of oxidative status, antioxidant defense systems and DNA damage, together with sperm parameters might be a useful tool for diagnosis and treatment of male infertility. FAU - Hosen, Md Bayejid AU - Hosen MB AD - Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka-1000, Bangladesh. FAU - Islam, Md Rakibul AU - Islam MR AD - Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka-1000, Bangladesh. FAU - Begum, Firoza AU - Begum F AD - Department of Obstetrics and Gynecology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka-1000, Bangladesh. FAU - Kabir, Yearul AU - Kabir Y AD - Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka-1000, Bangladesh.