• PMID: 26153379
• Gene Name: E330034G19Rik
• Condition: Role in spermatogenesis in mice
• Association: Associated
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: Role in spermatogenesis in mice

ZPAC is required for normal spermatogenesis in mice

Zuo EW, Yang XG, Lu YQ, Xie L, Shang JH, Li D, Yang H, Hu LL, Zhao HM, Lu SS, Lu KH.

The ubiquitin-proteasome pathway, involved in genetic recombination and sex-chromosome silencing during meiosis, plays critical roles in the specification of germ-line stem cells and the differentiation of gametes from gonocytes. Zygote-specific proteasome assembly chaperone (ZPAC) is expressed in the early mouse embryo, where it is important for progression of the mouse maternal-to-zygotic transition. The role of ZPAC during spermatogenesis in the adult gonads, however, remains unknown. In this study, rapid amplification of cDNA ends was used to determine the Zpac cDNA sequence, a 1584-bp transcript that includes a putative 1122-bp open reading frame coding for a 373 amino acid protein. Western blot and immunohistochemistry revealed that ZPAC was specifically expressed in gonads. To further dissect the function of ZPAC during spermatogenesis, we employed PiggyBac-based RNA interference vectors for transgenesis combined with cell transplantation to deplete Zpac during spermatogenesis. This RNAi-mediate depletion in Zpac expression disrupted normal spermatogenesis from spermatogonial stem cells. Two independent yeast two-hybrid screens further revealed an interaction between ZPAC and SYCE1. Together, these data suggest that ZPAC is required for normal spermatogenesis in mice. CI - © 2015 Wiley Periodicals, Inc. FAU - Zuo, Er-Wei AU - Zuo EW AD - State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi High Education Laboratory for Animal Reproduction and Biotechnology, Guangxi University, Nanning, Guangxi, 530004, China. FAU - Yang, Xiao-Gan AU - Yang XG AD - State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi High Education Laboratory for Animal Reproduction and Biotechnology, Guangxi University, Nanning, Guangxi, 530004, China. FAU - Lu, Yang-Qing AU - Lu YQ AD - State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi High Education Laboratory for Animal Reproduction and Biotechnology, Guangxi University, Nanning, Guangxi, 530004, China. AD - Regenerative Bioscience Center, Department of Animal and Dairy Science, University of Georgia, Athens, Georgia, 30602, USA. FAU - Xie, Long AU - Xie L AD - State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi High Education Laboratory for Animal Reproduction and Biotechnology, Guangxi University, Nanning, Guangxi, 530004, China. FAU - Shang, Jiang-Hua AU - Shang JH AD - Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning, Guangxi 530001, China. FAU - Li, Di AU - Li D AD - State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi High Education Laboratory for Animal Reproduction and Biotechnology, Guangxi University, Nanning, Guangxi, 530004, China. FAU - Yang, Huan AU - Yang H AD - State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi High Education Laboratory for Animal Reproduction and Biotechnology, Guangxi University, Nanning, Guangxi, 530004, China. FAU - Hu, Lin-Lin AU - Hu LL AD - State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi High Education Laboratory for Animal Reproduction and Biotechnology, Guangxi University, Nanning, Guangxi, 530004, China. FAU - Zhao, Hui-Min AU - Zhao HM AD - State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi High Education Laboratory for Animal Reproduction and Biotechnology, Guangxi University, Nanning, Guangxi, 530004, China. FAU - Lu, Sheng-Sheng AU - Lu SS AD - State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi High Education Laboratory for Animal Reproduction and Biotechnology, Guangxi University, Nanning, Guangxi, 530004, China.