• PMID: 26027266
• Gene Name: GPX1
• Condition: Male infertility
• Association: It has been established that imbalance in the glutathione system is the most prominent feature in men with infertility and overweight as compared to infertile men with normal body weight.
• Population size: 90
• Population details: 90 (60 men with infertility were examined--30 with normal weight and 30 with overweight), 30fertile men)
• Sex: Male
• Infertility type: Male infertility
• Associated genes: GSH, GR, GPO, GST
• Other associated phenotypes: Male infertility

[Glutathione-dependent enzymes and glutathione in infertility of men with different body mass]

Kolesnikova LI, Vanteeva OA, Kurashova NA, Dashiev BG.

OBJECTIVE: Our aim was to investigate the glutathione-disulfide system of antioxidant defense in men with different body mass index and infertility. METHODS: 60 men with infertility were examined--30 with normal weight and 30 with overweight. 30fertile men were included in the control group. Hemolysate made from red blood cells was used as a materialfor study. The level of reduced (GSH) and oxidized glutathione (GSSG), and glutathione activity was determined by enzyme spectrofluorophotometer SHIMADZU-1501 (Japan). RESULTS: In infertile men with normal body weight decrease of GSH to 15.35% was combined with a decrease in the activity of enzymes--glutathione reductase (GR) to 53.41%, glutathione peroxidase (GPO) to 51.44%, and increased activity of glutathione-S-transferaze (GST) to 31.52% as compared with the controlgroup. In the group of infertile men with overweight a decrease in the activity of GR, 56.77% and 56.22% at the GPO on the background of a stable level of GST was detected in comparison with fertile men. The concentration of GSH in infertile group decreased by 20.08%, and the concentration of GSSG increased by 20.38%. CONCLUSION: It has been established that imbalance in the glutathione system is the most prominent feature in men with infertility and overweight as compared to infertile men with normal body weight. FAU - Kolesnikova, L I AU - Kolesnikova LI FAU - Vanteeva, O A AU - Vanteeva OA FAU - Kurashova, N A AU - Kurashova NA