• PMID: 26004865
• Gene Name: PRPS2
• Condition: Sertoli-Cell Only Syndrome
• Association: PRPS2 expression correlates with Sertoli-cell only syndrome and inhibits the apoptosis of TM4 Sertoli cells via the p53/Bcl-2/caspases signaling pathway.
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: Sertoli-Cell Only Syndrome

PRPS2 Expression Correlates with Sertoli-Cell Only Syndrome and Inhibits the Apoptosis of TM4 Sertoli Cells

Lei B, Wan B, Peng J, Yang Y, Lv D, Zhou X, Shu F, Li F, Zhong L, Wu H, Mao X.

PURPOSE: Sertoli-cell only syndrome is one of the reasons for male infertility but its pathogenesis remains unclear. PRPS2, a subset of PRS, is reported to be a potential protein associated with Sertoli-cell only syndrome. In this study we further investigated the correlation between PRPS2 and Sertoli-cell only syndrome, and evaluated the effect of PRPS2 expression on apoptosis of TM4 Sertoli cells. MATERIALS AND METHODS: PRPS2 expression was detected in patients with Sertoli-cell only syndrome and normal spermatogenesis, and in Sertoli-cell only syndrome mouse models by immunohistochemistry, quantitative reverse transcription-polymerase chain reaction and Western blot. PRPS2 expression in TM4 Sertoli cells was then down-regulated and up-regulated by lentivirus vectors. The effect of PRPS2 expression on cell apoptosis and cell cycle transition was evaluated by flow cytometry. RESULTS: PRPS2 expression in patients with Sertoli-cell only syndrome was significantly greater than in those with normal spermatogenesis. A significant increase in PRPS2 expression was observed in Sertoli-cell only syndrome mouse models. PRPS2 over expression significantly inhibited cell apoptosis and promoted cell cycle transition in TM4 Sertoli cells. However, PRPS2 down-regulation showed a reverse effect. Moreover, results revealed that PRPS2 over expression inhibited cell apoptosis via the p53/Bcl-2/caspase-9/caspase-3/caspase-6/caspase-7 signaling pathway. CONCLUSIONS: PRPS2 expression correlates with Sertoli-cell only syndrome and inhibits the apoptosis of TM4 Sertoli cells via the p53/Bcl-2/caspases signaling pathway. CI - Copyright © 2015 American Urological Association Education and Research, Inc. Published by Elsevier Inc. All rights reserved. FAU - Lei, Bin AU - Lei B AD - Department of Urology, Peking University Shenzhen Hospital, Shenzhen, People's Republic of China; Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China. FAU - Wan, Bo AU - Wan B AD - Department of Urology, Peking University Shenzhen Hospital, Shenzhen, People's Republic of China; Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China. FAU - Peng, Jian AU - Peng J AD - Department of Urology, Peking University Shenzhen Hospital, Shenzhen, People's Republic of China. FAU - Yang, Yu AU - Yang Y AD - Department of Urology, Peking University Shenzhen Hospital, Shenzhen, People's Republic of China. FAU - Lv, Daojun AU - Lv D AD - Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China. FAU - Zhou, Xuming AU - Zhou X AD - Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China. FAU - Shu, Fangpeng AU - Shu F AD - Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China. FAU - Li, Fei AU - Li F AD - Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China. FAU - Zhong, Liren AU - Zhong L AD - Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China. FAU - Wu, Huayan AU - Wu H AD - Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China.