• PMID: 25264968
• Gene Name: PON1
• Condition: Male infertility
• Association: The study speculates that the low activity of PON1 can be a risk factor for male infertility probably due to a decrease in antioxidant activity of PON1 and increase in lipid peroxidation.
• Mutation: Q192R variants of PON1
• Population size: 300
• Population details: 300 (150 fertile, 150 infertile men )
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: Male infertility

Genotype and phenotype frequencies of paraoxonase 1 in fertile and infertile men

Tavilani H, Fattahi A, Esfahani M, Khodadadi I, Karimi J, Bahrayni E, Vatannejad A, Vaisi-Raygani A, Ghorbani M, Latifi Z.

Paraoxonase1 (PON1) is a glycoprotein associated with high density lipoprotein and has antioxidant activity. The impact of PON1 in various stages of spermatogenesis has also been suggested. This study was aimed to investigate frequencies of phenotypes and Q192R genotypes of PON1 in fertile and infertile males. Q192R variants of PON1 were determined in 150 fertile and 150 infertile men using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Plasma arylesterase and paraoxonase activities were detected by spectrophotometry and malondialdehyde (MDA) level was measured using thiobarbituric acid. Our results showed no significant difference in the distribution of PON1 genotypes and alleles between fertile and infertile groups. However morphology and motility of sperm were associated with various genotypes of PON1. The number of fertile males with the BB phenotype (high activity) was significantly higher than that of infertile males, whereas the number of individuals with the AB phenotype (moderate activity) was statistically higher in infertile men compared with the fertile group. Additionally, MDA and arylesterase activity levels were significantly higher in infertile subjects compared with fertile men. We speculate that the low activity of PON1 can be a risk factor for male infertility probably due to a decrease in antioxidant activity of PON1 and increase in lipid peroxidation. FAU - Tavilani, Heidar AU - Tavilani H AD - Urology and Nephrology Research Center, Hamadan University of Medical Sciences , Hamadan , Iran . FAU - Fattahi, Amir AU - Fattahi A FAU - Esfahani, Maryam AU - Esfahani M FAU - Khodadadi, Iraj AU - Khodadadi I FAU - Karimi, Jamshid AU - Karimi J FAU - Bahrayni, Elham AU - Bahrayni E FAU - Vatannejad, Akram AU - Vatannejad A FAU - Vaisi-Raygani, Asad AU - Vaisi-Raygani A FAU - Ghorbani, Marzyeh AU - Ghorbani M