• PMID: 23869807
• Gene Name: CCNA1
• Condition: Non obstructive azoospermia, Sertoli cell only syndrome
• Association: Associated
• Population size: 20
• Population details: 20 (4 controls, 16 cases)
• Sex: Male
• Infertility type: Male infertility
• Associated genes: GSE45887 analyzed by GEO2R (cutoff 1.5 fold)

The gene expression analysis of paracrine/autocrine factors in patients with spermatogenetic failure compared with normal spermatogenesis

Malcher A, Rozwadowska N, Stokowy T, Jedrzejczak P, Zietkowiak W, Kurpisz M.

PROBLEM: The aim of this study was to examine the expression levels of IL-1 family members, IL-6, IL-10, TNF family, SCF, and c-kit in infertile patients with idiopathic non-obstructive azoospermia (NOA) compared with men with normal spermatogenesis. METHOD OF STUDY: We analyzed 20 testicular biopsy samples with Affymetrix Human Gene 1.0 ST microarrays (Affymetrix, Santa Clara, CA, USA). Sixteen of them were obtained from patients with various types of NOA and four with normal spermatogenesis. RESULTS: The comparative analysis of normal and pathological group demonstrated a different expression level of IL1-RA gene. It was also observed that the gene expression levels for IL1-R1, CASP1, and stem cell factor (SCF) were upregulated in the Sertoli-cell-only syndrome group in comparison with the control one (P < 0.05). CONCLUSION: The microarray analysis showed the expression level of all investigated paracrine/autocrine factors at one go, and therefore, the possible interaction between these genes could be examined. CI - © 2013 John Wiley & Sons Ltd. FAU - Malcher, Agnieszka AU - Malcher A AD - Department of Reproductive Biology and Stem Cells, Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland. FAU - Rozwadowska, Natalia AU - Rozwadowska N FAU - Stokowy, Tomasz AU - Stokowy T FAU - Jedrzejczak, Piotr AU - Jedrzejczak P FAU - Zietkowiak, Wojmir AU - Zietkowiak W