• PMID: 23641067
• Gene Name: CETN1
• Condition: Male infertility
• Association: Associated
• Sex: Male
• Infertility type: Male infertility

Germline deletion of Cetn1 causes infertility in male mice

Avasthi P, Scheel JF, Ying G, Frederick JM, Baehr W, Wolfrum U.

Centrins are calmodulin-like Ca(2+)-binding proteins that can be found in all ciliated eukaryotic cells from yeast to mammals. Expressed in male germ cells and photoreceptors, centrin 1 (CETN1) resides in the photoreceptor transition zone and connecting cilium. To identify its function in mammals, we deleted Cetn1 by homologous recombination. Cetn1(-/-) mice were viable and showed no sign of retina degeneration suggesting that CETN1 is nonessential for photoreceptor ciliogenesis or structural maintenance. Phototransduction components localized normally to the Cetn1(-/-) photoreceptor outer segments, and loss of CETN1 had no effect on light-induced translocation of transducin to the inner segment. Although Cetn1(-/-) females and Cetn1(+/-) males had normal fertility, Cetn1(-/-) males were infertile. The Cetn1(-/-) testes size was normal, and spermatogonia as well as spermatocytes developed normally. However, spermatids lacked tails suggesting severe defects at the late maturation phase of spermiogenesis. Viable sperm cells were absent and the few surviving spermatozoa were malformed. Light and electron microscopy analyses of Cetn1(-/-) spermatids revealed failures in centriole rearrangement during basal body maturation and in the basal-body-nucleus connection. These results confirm an essential role for CETN1 in late steps of spermiogenesis and spermatid maturation. FAU - Avasthi, Prachee AU - Avasthi P AD - Department of Ophthalmology, University of Utah Health Science Center, 65 Mario Capecchi Drive, Salt Lake City, UT 84132, USA. FAU - Scheel, Jan Frederik AU - Scheel JF FAU - Ying, Guoxin AU - Ying G FAU - Frederick, Jeanne M AU - Frederick JM FAU - Baehr, Wolfgang AU - Baehr W