• PMID: 23519396
• Gene Name: CATSPER2
• Condition: Oligoasthenozoospermic
• Association: Associated
• Sex: Male
• Infertility type: Male infertility
• Associated genes: CatSper 2, Tektin 2
• Other associated phenotypes: Oligoasthenozoospermic

Levels of Tektin 2 and CatSper 2 in normozoospermic and oligoasthenozoospermic men and its association with motility, fertilization rate, embryo quality and pregnancy rate

Bhilawadikar R, Zaveri K, Mukadam L, Naik S, Kamble K, Modi D, Hinduja I.

PURPOSE: To compare the expression profiles of Tektin 2 and CatSper 2 motility proteins in the spermatozoa of normozoospermic and oligoasthenozoospermic men and determine its correlation with sperm motility, fertilization rate, embryo quality and pregnancy rate. METHODS: Tektin 2 and CatSper 2 protein expression was studied using Western Blotting and immunofluorescence. Tektin 2 and CatSper 2 protein levels were quantified by ELISA. RESULTS: Oligoasthenozoospermic men were found to have lower fertilization rates, poor embryo quality and lower pregnancy rates as compared to normozoospermic men. The levels of Tektin 2 and CatSper 2 are significantly lower in spermatozoa of oligoasthenozoospermic men as compared to normozoospermic controls; the levels were also lower in immotile fraction as compared to motile fraction of spermatozoa obtained from normozoospermic individuals. The levels of Tektin 2 and CatSper 2 were higher in individuals demonstrating sperm motility >60 % as compared to sperm motility <30 %. Tektin 2 but not CatSper 2 levels were positively associated with fertilization rate, embryo quality and pregnancy rate. CONCLUSION: Levels of Tektin 2 and CatSper 2 proteins are positively associated with sperm motility parameters. Measurements of Tektin 2 levels can be correlated with the clinical outcome of ICSI. FAU - Bhilawadikar, Rashmi AU - Bhilawadikar R AD - Jaslok Hospital, 15, Dr G Deshmukh Marg, Mumbai - 26, India. FAU - Zaveri, Kusum AU - Zaveri K FAU - Mukadam, Leena AU - Mukadam L FAU - Naik, Shilpa AU - Naik S FAU - Kamble, Ketan AU - Kamble K FAU - Modi, Deepak AU - Modi D