• PMID: 23455884
• Gene Name: GPX4
• Condition: Idiopathic asthenozoospermia
• Association: Sixteen proteins identified belonged to 15 unique protein groups. GRP78, lactoferrin, SPANXB, PGK2, flagellin, DJ-1, XPA binding protein 2, CAB2, GPX4, and GAPDH were the first to be identified as differentially expressed proteins in idiopathic asthenospe
• Sex: Male
• Infertility type: Male infertility
• Associated genes: GRP78, lactoferrin, SPANXB, PGK2, flagellin, DJ-1, XPA binding protein 2, CAB2, GPX4, and GAPDH
• Other associated phenotypes: Idiopathic asthenozoospermia

Comparative proteomic study between human normal motility sperm and idiopathic asthenozoospermia

Shen S, Wang J, Liang J, He D.

PURPOSE: Idiopathic asthenozoospermia is considered as one of the causes of male infertility and characterized by reduced sperm motility. For a better determination of pathogenic mechanism of asthenozoospermia, the exploration of differentially expressed proteins in normal sperm motility and idiopathic asthenozoospermia was conducted in our study. METHODS: Sperm proteins were extracted and isolated by two-dimensional electrophoresis. All significantly changed protein spots were picked up from 2D gels and identified by tandem mass spectrometry. Sixteen of the thirty-three total differentially expressed protein spots were successfully identified by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry. RESULTS: Sixteen proteins identified belonged to 15 unique protein groups. GRP78, lactoferrin, SPANXB, PGK2, flagellin, DJ-1, XPA binding protein 2, CAB2, GPX4, and GAPDH were the first to be identified as differentially expressed proteins in idiopathic asthenospermia patients. Meanwhile, the analysis of quantitative RT-PCR was carried out to compare the protein levels, and the results indicated that the expression levels of the gene and protein were not entirely consistent. CONCLUSIONS: These experimental results expand the scope of the protein database, generating targets for further investigation of the pathogenic mechanism of idiopathic asthenozoospermia. FAU - Shen, Shulin AU - Shen S AD - Department of Andrology, Liuzhou Hospital of Traditional Chinese Medicine, Liuzhou, 545001, China. FAU - Wang, Jinzi AU - Wang J FAU - Liang, Jihong AU - Liang J