• PMID: 23174138
• Gene Name: TUBB
• Condition: Idiopathic asthenozoospermia, male infertility
• Association: The results together assume that a cytoskeletal reorganization induced by a disturbance in the physicochemical features of sperm plasma membrane, and potentially mediated by ezrin, Cdc42, and tetraspanin CD9, could have a role in idiopathic asthenozoosper
• Population size: 46
• Population details: 46 (25 infertile patients affected by idiopathic asthenozoospermia, 21 age-matched normospermic fertile donors)
• Sex: Male
• Infertility type: Male infertility
• Associated genes: Ezrin, Cdc42, CD9, F-actin, and ?-tubulin
• Other associated phenotypes: Idiopathic asthenozoospermia, male infertility

Involvement of sperm plasma membrane and cytoskeletal proteins in human male infertility

Salvolini E, Buldreghini E, Lucarini G, Vignini A, Lenzi A, Di Primio R, Balercia G.

OBJECTIVE: To assess the physicochemical characteristics of sperm plasma membrane and to evaluate the immunohistochemical expression of transmembrane and cytoskeletal proteins in spermatozoa isolated from normospermic fertile donors and asthenozoospermic infertile patients. DESIGN: Case-control study. SETTING: Academic male infertility center. PATIENT(S): Twenty-five infertile patients affected by idiopathic asthenozoospermia and 21 age-matched normospermic fertile donors. INTERVENTION(S): Sperm parameters were evaluated; membrane fluidity and hydration studies, and immunohistochemical analysis were performed in isolated spermatozoa. MAIN OUTCOME MEASURE(S): Semen analyses to ascertain volume, sperm count, motility, and morphology; then membrane fluidity and hydration studies and immunohistochemical analysis were performed on isolated spermatozoa. RESULT(S): Spermatozoa from the asthenozoospermic group exhibited a reduced fluidity at the lipid-water interface level, an increased fluidity of the deeper portion of the bilayer, and a lower plasma membrane hydration than normospermic cells. Moreover, the immunohistochemical expression of ezrin, Cdc42, CD9, F-actin, and β-tubulin was higher in normospermic samples. CONCLUSION(S): Our results together assume that a cytoskeletal reorganization induced by a disturbance in the physicochemical features of sperm plasma membrane, and potentially mediated by ezrin, Cdc42, and tetraspanin CD9, could have a role in idiopathic asthenozoospermia. CI - Copyright © 2013 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved. FAU - Salvolini, Eleonora AU - Salvolini E AD - Department of Clinical and Molecular Sciences-Histology, Polytechnic University of Marche, Ancona, Italy. e.salvolini@univpm.it FAU - Buldreghini, Eddi AU - Buldreghini E FAU - Lucarini, Guendalina AU - Lucarini G FAU - Vignini, Arianna AU - Vignini A FAU - Lenzi, Andrea AU - Lenzi A FAU - Di Primio, Roberto AU - Di Primio R