• PMID: 22825968
• Gene Name: BMPR2
• Condition: PCOS
• Association: BMP15 and GDF9 expression per oocyte was higher among women with PCOS than the control group. A positive correlation was found between BMPR2 transcripts and hyperandrogenism in FF of PCOS patients. Progesterone values in FF were lower in the PCOS group.
• Population size: 53
• Population details: 53 (18 patients with PCOS, 35 controls)
• Sex: Male
• Infertility type: Male infertility
• Associated genes: BMPR2, BMP15, and GDF9
• Other associated phenotypes: PCOS

Single-cell expression analysis of BMP15 and GDF9 in mature oocytes and BMPR2 in cumulus cells of women with polycystic ovary syndrome undergoing controlled ovarian hyperstimulation

de Resende LO, Vireque AA, Santana LF, Moreno DA, de Sá Rosa e Silva AC, Ferriani RA, Scrideli CA, Reis RM.

PURPOSE: To detect expression of bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9) in oocytes, and their receptor type 2 receptor for BMPs (BMPR2) in cumulus cells in women with polycystic ovary syndrome (PCOS) undergoing in vitro fertilization (IVF), and determine if BMPR2, BMP15, and GDF9 expression correlate with hyperandrogenism in FF of PCOS patients. METHODS: Prospective case-control study. Eighteen MII-oocytes and their respective cumulus cells were obtained from 18 patients with PCOS, and 48 MII-oocytes and cumulus cells (CCs) from 35 controls, both subjected to controlled ovarian hyperstimulation (COH), and follicular fluid (FF) was collected from small (10-14 mm) and large (>18 mm) follicles. RNeasy Micro Kit (Qiagen) was used for RNA extraction and gene expression was quantified in each oocyte individually and in microdissected cumulus cells from cumulus-oocyte complexes retrieved from preovulatory follicles using qRT-PCR. Chemiluminescence and RIA assays were used for hormone assays. RESULTS: BMP15 and GDF9 expression per oocyte was higher among women with PCOS than the control group. A positive correlation was found between BMPR2 transcripts and hyperandrogenism in FF of PCOS patients. Progesterone values in FF were lower in the PCOS group. CONCLUSION: We inferred that BMP15 and GDF9 transcript levels increase in mature PCOS oocytes after COH, and might inhibit the progesterone secretion by follicular cells in PCOS follicles, preventing premature luteinization in cumulus cells. BMPR2 expression in PCOS cumulus cells might be regulated by androgens. FAU - de Resende, Luciana Ochuiuto Teixeira AU - de Resende LO AD - Department of Gynecology and Obstetrics - Faculty of Medicine of Ribeirão Preto, University of São Paulo, São Paulo, Brazil. FAU - Vireque, Alessandra Aparecida AU - Vireque AA FAU - Santana, Laura Ferreira AU - Santana LF FAU - Moreno, Daniel Antunes AU - Moreno DA FAU - de Sá Rosa e Silva, Ana Carolina Japur AU - de Sá Rosa e Silva AC FAU - Ferriani, Rui Alberto AU - Ferriani RA FAU - Scrideli, Carlos Alberto AU - Scrideli CA