• PMID: 22154371
• Gene Name: SOD
• Condition: Sperm motility, Male infertility
• Association: The positive correlation between post-thaw motility recovery and SOD content in mature spermatozoa provides a good predictor of post-thaw motility recovery after cryopreservation.
• Population size: 42
• Population details: 42 normozoospermic patients consulting for infertility
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: Sperm motility, Male infertility

Superoxide dismutase content in sperm correlates with motility recovery after thawing of cryopreserved human spermatozoa

Buffone MG, Calamera JC, Brugo-Olmedo S, De Vincentiis S, Calamera MM, Storey BT, Doncel GF, Alvarez JG.

OBJECTIVE: To investigate the correlation between sperm superoxide dismutase (SOD) content and motility recovery after thawing of cryopreserved human sperm, based on the rationale that this antioxidant enzyme provides protection against reactive oxygen species-induced damage during cryopreservation. DESIGN: Prospective study. SETTING: Private infertility institute and university-based research laboratory. PATIENT(S): Forty-two consenting normozoospermic patients consulting for infertility. INTERVENTION(S): The SOD content was measured in sperm from unfractionated samples and in sperm recovered from the pellet fraction obtained after discontinuous density gradient centrifugation. MAIN OUTCOME MEASURE(S): Sperm motility was evaluated post-thaw in the two sets of samples and motility recovery was plotted against the sperm SOD content to determine their correlation. RESULT(S): There was a significant positive correlation between motility recovery after thawing and SOD content in sperm from the 90% gradient pellet containing highly purified mature sperm. There was also a significant negative correlation between motility after thawing and SOD content in the unfractionated sample. CONCLUSION(S): The positive correlation between post-thaw motility recovery and SOD content in mature spermatozoa provides a good predictor of post-thaw motility recovery after cryopreservation. CI - Copyright © 2012 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved. FAU - Buffone, Mariano G AU - Buffone MG AD - Instituto de Biologia y Medicina Experimental, CONICET, Buenos Aires, Argentina. FAU - Calamera, Juan C AU - Calamera JC FAU - Brugo-Olmedo, Santiago AU - Brugo-Olmedo S FAU - De Vincentiis, Sabrina AU - De Vincentiis S FAU - Calamera, Maria M AU - Calamera MM FAU - Storey, Bayard T AU - Storey BT FAU - Doncel, Gustavo F AU - Doncel GF