• PMID: 21575935
• Gene Name: PARK7
• Condition: Asthenozoospermia
• Association: DJ-1 levels are reduced in moderate asthenozoospermia patients. DJ-1 concentration is positively correlated with sperm motility and sperm SOD activity indicated by partial correlation analysis.
• PMID: 21575935
• Gene Name: PARK7
• Condition: Asthenozoospermia, Male infertility
• Association: The sperm DJ-1 concentration in moderate asthenozoospermia patients was lower than those in mild asthenozoospermia patients and control subjects. DJ-1 with a more acidic pI was increased in asthenozoospermia patients. Sperm SOD activity was decreased in a
• Population size: 184
• Population details: 184 (113 asthenozoospermia patients, 70 mild asthenozoospermia patients, 43 moderate asthenozoospermia patients, 58 age-matched controls)
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: Asthenozoospermia, Male infertility

Down-regulation of DJ-1 protein in the ejaculated spermatozoa from Chinese asthenozoospermia patients

An CN, Jiang H, Wang Q, Yuan RP, Liu JM, Shi WL, Zhang ZY, Pu XP.

OBJECTIVE: To determine whether the expression of DJ-1 protein, whose levels in spermatozoa have been reported to be highly correlated with male infertility caused by toxicants, is changed in spermatozoa of Chinese asthenozoospermia patients. DESIGN: DJ-1 measurement by Western blotting, quantitive ELISA, and isoelectric-focusing electrophoresis (IFE) combined with immunoblotting. SETTING: Academic medical center and research laboratories. PATIENT(S): Asthenozoospermia patients (n = 113), including mild asthenozoospermia patients (n = 70) and moderate asthenozoospermia patients (n = 43), and age-matched control subjects (n = 58). INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): DJ-1 in spermatozoa was determined by Western blotting and ELISA, the isoelectric point (pI) of DJ-1 by IFE combined with immunoblotting, and sperm superoxide dismutase (SOD) activity by an assay kit. RESULT(S): The sperm DJ-1 concentration in moderate asthenozoospermia patients was lower than those in mild asthenozoospermia patients and control subjects. DJ-1 with a more acidic pI was increased in asthenozoospermia patients. Sperm SOD activity was decreased in asthenozoospermia patients. CONCLUSION(S): DJ-1 levels are reduced in moderate asthenozoospermia patients. DJ-1 concentration is positively correlated with sperm motility and sperm SOD activity indicated by partial correlation analysis. CI - Copyright © 2011 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved. FAU - An, Chun-Na AU - An CN AD - State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Science, Peking University, People's Republic of China. FAU - Jiang, Hui AU - Jiang H FAU - Wang, Qi AU - Wang Q FAU - Yuan, Ren-Pei AU - Yuan RP FAU - Liu, Jian-Ming AU - Liu JM FAU - Shi, Wan-Lu AU - Shi WL FAU - Zhang, Zi-Yu AU - Zhang ZY