• PMID: 21219384
• Gene Name: CCNA2
• Condition: Nonobstructive azoospermia, hypospermatogenesis, male infertility
• Association: Associated
• Population size: 63
• Population details: 63 (48 infertile males (nonobstructive azoospermia), 15 normal subjects)
• Sex: Male
• Infertility type: Male infertility
• Associated genes: Cyclin A
• Other associated phenotypes: Nonobstructive azoospermia, hypospermatogenesis, male infertility

Immunohistochemical expression of cyclin A in testicular biopsies of fertile and infertile men: correlation with the morphometry of seminiferous tubules

Abdou AG, Hammam MA, Farag AG, Farouk S, Fawzy M.

Cyclin A is a member of the cyclin family of proteins, which are required for both the mitotic and meiotic divisions that characterise spermatogenesis in human and other mammalian species. The data on cyclin A expression in various human spermatogenic disorders and its relationship to the morphology of seminiferous tubules are not well clarified. This study aimed to evaluate the immunohistochemical expression of cyclin A in testicular biopsies of different spermatogenic disorders correlating with the morphology of seminiferous tubules using morphometry tools. Immunohistochemical evaluation of cyclin A was carried out on testicular biopsies obtained from 48 infertile males (nonobstructive azoospermia) and 15 normal subjects together with using semiautomatic morphometric analysis for evaluation of seminiferous tubules. Cyclin A is expressed in 100% of normal and hypospermatogenesis groups and in 80% of maturation arrest group, with complete absence in Sertoli cell only group. In positive cases, cyclin A stained the nuclei of spermatogonia and primary spermatocytes with a higher intensity of expression in normal cases compared with infertile group. Cyclin A expression was significantly associated with the different examined morphometric parameters. Cyclin A is involved in both mitosis and meiosis of human spermatogenesis as it is expressed in spermatogonia and primary spermatocytes. Morphometry of human testis is intimately correlated with the testicular histopathology. CI - © 2010 Blackwell Verlag GmbH. FAU - Abdou, A G AU - Abdou AG AD - Pathology Department, Menofiya University, Shebein Elkom, Egypt. Asmaa_elsaidy@yahoo.com FAU - Hammam, M A AU - Hammam MA FAU - Farag, A G A AU - Farag AG FAU - Farouk, S AU - Farouk S