• PMID: 20724452
• Gene Name: HSF2
• Condition: Assocaited with male germ cell maturation, spermatogenesis
• Association: Associated
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: Assocaited with male germ cell maturation, spermatogenesis

miR-18, a member of Oncomir-1, targets heat shock transcription factor 2 in spermatogenesis

Björk JK, Sandqvist A, Elsing AN, Kotaja N, Sistonen L.

miR-18 belongs to the Oncomir-1 or miR-17~92 cluster that is intimately associated with the occurrence and progression of different types of cancer. However, the physiological roles of the Oncomir-1 cluster and its individual miRNAs are largely unknown. Here, we describe a novel function for miR-18 in mouse. We show that miR-18 directly targets heat shock factor 2 (HSF2), a transcription factor that influences a wide range of developmental processes including embryogenesis and gametogenesis. Furthermore, we show that miR-18 is highly abundant in testis, displaying distinct cell-type-specific expression during the epithelial cycle that constitutes spermatogenesis. Expression of HSF2 and of miR-18 exhibit an inverse correlation during spermatogenesis, indicating that, in germ cells, HSF2 is downregulated by miR-18. To investigate the in vivo function of miR-18 we developed a novel method, T-GIST, and demonstrate that inhibition of miR-18 in intact seminiferous tubules leads to increased HSF2 protein levels and altered expression of HSF2 target genes. Our results reveal that miR-18 regulates HSF2 activity in spermatogenesis and link miR-18 to HSF2-mediated physiological processes such as male germ cell maturation. FAU - Björk, Johanna K AU - Björk JK AD - Department of Biosciences, Abo Akademi University, Turku, Finland. FAU - Sandqvist, Anton AU - Sandqvist A FAU - Elsing, Alexandra N AU - Elsing AN FAU - Kotaja, Noora AU - Kotaja N