• PMID: 20538714
• Gene Name: BRDT
• Condition: Subfertility associated with impaired sperm chromatin condensation
• Association: Associated
• Sex: Male
• Infertility type: Male infertility
• Associated genes: BRDT
• Other associated phenotypes: Subfertility associated with impaired sperm chromatin condensation

The interaction of modified histones with the bromodomain testis-specific (BRDT) gene and its mRNA level in sperm of fertile donors and subfertile men

Steilmann C, Cavalcanti MC, Bartkuhn M, Pons-Kühnemann J, Schuppe HC, Weidner W, Steger K, Paradowska A.

As histone modifications have been suggested to be involved in the regulation of gene expression after fertilisation, the present study aimed to analyze the interaction between the bromodomain testis-specific (BRDT) gene and differentially modified histones in human spermatozoa. The BRDT transcript level was studied to identify possible correlations between epigenetic changes, mRNA level and subfertility associated with impaired sperm chromatin condensation. Chromatin immunoprecipitation (ChIP) was performed with ejaculates from fertile and subfertile men using antibodies against specifically acetylated and methylated histone H3. Immunoprecipitated DNA was analysed by real-time quantitative PCR with primer pairs for BRDT. The BRDT mRNA level was screened by real-time RT-PCR. ChIP assay revealed co-localisation of acetylated and methylated histones within promoter and exon regions of the BRDT gene in fertile men. Interestingly, reduced binding of investigated modified histone modifications was observed in the BRDT promoter of subfertile patients. Different mRNA levels of BRDT have been detected in a group of infertile patients, as well as in fertile men. Enrichment of methylated histones within the BRDT promoter of fertile sperm suggests that this epigenetic mark may cause repression of BRDT after fertilisation, and may be changed in infertile patients. Our data suggest that reduced histone methylation in the promoter of BRDT may be associated with increased transcript levels in subfertile patients. FAU - Steilmann, Cornelia AU - Steilmann C AD - Department of Urology, Pediatric Urology and Andrology, Rudolf Buchheim-Strasse 7, Justus Liebig University, 35385 Giessen, Germany. cornelia.steilmann@chiru.med.uni-giessen.de FAU - Cavalcanti, Márcia C O AU - Cavalcanti MC FAU - Bartkuhn, Marek AU - Bartkuhn M FAU - Pons-Kühnemann, Jörn AU - Pons-Kühnemann J FAU - Schuppe, Hans-Christian AU - Schuppe HC FAU - Weidner, Wolfgang AU - Weidner W FAU - Steger, Klaus AU - Steger K