• PMID: 20488182
• Gene Name: CREM
• Condition: Associated with gene transcription dring spermatogenesis
• Association: Associated
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: Associated with gene transcription dring spermatogenesis

Sperm associated antigen 8 (SPAG8), a novel regulator of activator of CREM in testis during spermatogenesis

Wu H, Chen Y, Miao S, Zhang C, Zong S, Koide SS, Wang L.

cAMP response element modulator (CREM)-mediated gene expression is an essential regulatory mechanism for germ cell differentiation. CREM and its coactivator in testis, ACT, activate the transcription of many essential genes for spermatogenesis. Sperm associated antigen 8 (SPAG8) is a testis-specific component that is expressed during germ cell differentiation. In this study, we found the pattern of SPAG8 expression largely overlapped with that of ACT during spermatogenesis and verified the association of SPAG8 with ACT. Furthermore, we showed that SPAG8 enhanced the transcriptional activation of ACT-mediated CREMtau by strengthening the binding of ACT to CREMtau. These results indicate that SPAG8 acts as a regulator of ACT and plays an important role in CREM-ACT-mediated gene transcription during spermatogenesis. CI - Copyright 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. FAU - Wu, Hongyu AU - Wu H AD - National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Tsinghua University, Beijing, China. FAU - Chen, Yingchun AU - Chen Y FAU - Miao, Shiying AU - Miao S FAU - Zhang, Changyong AU - Zhang C FAU - Zong, Shudong AU - Zong S FAU - Koide, Samuel S AU - Koide SS