• PMID: 20407872
• Gene Name: Syce3
• Condition: May participate in the apoptosis of spermatogenic cells and the pathogenesis of cryptorchidism
• Association: Associated
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: May participate in the apoptosis of spermatogenic cells and the pathogenesis of cryptorchidism

Expression pattern of testis-specific expressed gene 2 in cryptorchidism model and its role in apoptosis of spermatogenic cells

Hu T, Wang Z, Zeng F, Chen X, Gu Z, Zheng L, Tong Q.

In our previous study, we identified a novel testis-specific expressed gene 2 (TSEG-2) from mouse testis. To further investigate its functions, 35 male Balb/c mice (8 weeks old) were divided into cryptorchidism group (n=20), sham group (n=10), and control group (n=5). In cryptorchidism group, the right testes were anchored to the inner lateral abdominal wall. In situ hybridization (ISH) was applied to measure the localization of TSEG-2 in mouse testis. Real-time quantitative PCR was performed to detect the expression of TSEG-2 gene. Meanwhile, under the mediation of polyethylenimine (PEI), the recombinant vector pEGFP-TSEG-2 (n=5) or empty vector (mock, n=5) was transfected into the testis of male mice. The transfection efficiencies were measured under a fluorescence microscope. The apoptosis of spermatogenic cells was detected by terminal deoxynuleotidyl-mediated nick end labeling (TUNEL). The results showed that TSEG-2 was expressed in convoluted seminiferous tubules, more precisely, in spermatogonia and spermatocytes. As compared with sham and control groups, the TSEG-2 transcription was significantly enhanced (P<0.05) and was correlated with apoptosis of spermatogenic cells in cryptorchid testes (P<0.05). PEI was efficient in mediating transfection of TSEG-2 into seminiferous tubules of testis. One week post-transfection, intratesticular injection of TSEG-2 resulted in increased apoptosis of spermatogenic cells in vivo (P<0.05). These results indicate that TSEG-2 may participate in the apoptosis of spermatogenic cells and the pathogenesis of cryptorchidism. FAU - Hu, Tao AU - Hu T AD - Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. hutao19771212@163.com FAU - Wang, Zhiyu AU - Wang Z FAU - Zeng, Fuqing AU - Zeng F FAU - Chen, Xiaochun AU - Chen X FAU - Gu, Zhaohui AU - Gu Z FAU - Zheng, Liduan AU - Zheng L