• PMID: 19288454
• Gene Name: CAT
• Condition: Male infertility
• Association: In conclusion, the results suggest that the evaluation of oxidative status and antioxidant defenses may be taken as an important tool for diagnosis and treatment of male infertility.
• Population size: 102
• Population details: 102 (9 normozoospermic, 13 azoospermic, 38 oligoasthenozoospermic, 42 asthenozoospermic )
• Sex: Male
• Infertility type: Male infertility
• Associated genes: CAT, SOD
• Other associated phenotypes: Male infertility

Lipid peroxidation and antioxidant enzyme activities in infertile men: correlation with semen parameter

Ben Abdallah F, Dammak I, Attia H, Hentati B, Ammar-Keskes L.

The aim of this study was to investigate the oxidative stress status and antioxidant enzyme activities in infertile men's semen and to determine their relationship with spermatozoa characteristics. Four groups of infertile men, normozoospermic (n=9), azoospermic (n=13), oligoasthenozoospermic (n=38), and asthenozoospermic (n=42), were tested for malonaldialdehyde (MDA) concentration and catalase (CAT) and superoxide dismutase (SOD) activities in semen using spectrophotometric methods. We found that MDA levels in semen and SOD activity in seminal plasma (SP) were significantly higher in oligoasthenozoospermic and asthenozoospermic groups compared with normozoospermic group. In contrast, the mean values of CAT activity were not significantly different in all groups. We found positive correlations between semen MDA concentration and SOD activity (P<0.01). MDA levels in sperm pellet and in SP were also positively correlated with mobility grade b (P<0.01), acrosome anomalies (P<0.01), and residual cytoplasmic droplets (P<0.05). In contrast, SOD activity in SP was negatively correlated with sperm concentration and normal morphology (P<0.05). Similarly, CAT activity in SP was negatively correlated with sperm and leukocyte concentrations (P<0.05). In conclusion, our results suggest that the evaluation of oxidative status and antioxidant defenses may be taken as an important tool for diagnosis and treatment of male infertility. CI - (c) 2009 Wiley-Liss, Inc. FAU - Ben Abdallah, Fatma AU - Ben Abdallah F AD - Human Pathologies and Oxidative Stress Unit, Superior Institute of Biotechnology of Sfax, Sfax, Tunisia. fatma_isbs@yahoo.fr FAU - Dammak, Ines AU - Dammak I FAU - Attia, Hamadi AU - Attia H FAU - Hentati, Basma AU - Hentati B