• PMID: 19271754
• Gene Name: CABS1
• Condition: Involved in the extremely complex structural rearrangements occurring in haploid germ cells during spermiogenesis
• Association: Associated
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: Involved in the extremely complex structural rearrangements occurring in haploid germ cells during spermiogenesis

CLPH, a novel casein kinase 2-phosphorylated disordered protein, is specifically associated with postmeiotic germ cells in rat spermatogenesis

Calvel P, Kervarrec C, Lavigne R, Vallet-Erdtmann V, Guerrois M, Rolland AD, Chalmel F, Jégou B, Pineau C.

In a recent proteomic study of rat spermatogenesis, we identified CLPH (for Casein-Like PHosphoprotein), a new testis-specific protein expressed exclusively in postmeiotic germ cells. In situ hybridization showed that the CLPH transcript was mainly present in round spermatids, whereas the protein was specifically detected by immunohistochemistry in elongated spermatids and in residual bodies. Electron microscopy showed the protein to be mostly cytoplasmic, but also frequently associated with the mitochondrial inner membrane during the last steps of spermatid differentiation. The Clph gene was found to be present solely in mammalian genomes, in a chromosomal region syntenic to the mammalian cluster of secretory calcium-binding phosphoprotein (SCPP) genes. CLPH has several distinctive properties in common with SCPPs: calcium overlay experiments showed that CLPH was a calcium-binding protein, whereas trypsin digestion assay, circular dichroism and fluorescence experiments demonstrated its intrinsically disordered structure. We also showed that CLPH was phosphorylated in vitro and in vivo by casein kinase 2, an enzyme critical for spermatid elongation. Given the specific and strong production of CLPH during rat spermiogenesis, together with the particular biochemical properties of this protein, we suggest that CLPH is involved in the extremely complex structural rearrangements occurring in haploid germ cells during spermiogenesis. FAU - Calvel, Pierre AU - Calvel P AD - Inserm, U625, Rennes, Universite Rennes I, Campus de Beaulieu, IFR-140, GERHM, Rennes, F-35042, France. FAU - Kervarrec, Christine AU - Kervarrec C FAU - Lavigne, Régis AU - Lavigne R FAU - Vallet-Erdtmann, Virginie AU - Vallet-Erdtmann V FAU - Guerrois, Myriam AU - Guerrois M FAU - Rolland, Antoine D AU - Rolland AD FAU - Chalmel, Frédéric AU - Chalmel F FAU - Jégou, Bernard AU - Jégou B