• PMID: 18488337
• Gene Name: CALM1
• Condition: Role n sperm capacitation
• Association: Associated
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: Role n sperm capacitation

[Calmodulin antagonists are involved in mouse sperm capacitation]

Li K, Xue YM, Lu JQ.

OBJECTIVE: To investigate the possible involvement of calmodulin in mouse sperm capacitation. METHODS: Calmodulin antagonists W7 at the concentrations of 50, 100 and 200 micromol/L and calmidazolium (CZ) at the concentrations of 10, 20 and 30 micromol/ L, were coincubated with mouse sperm for 2 hours, respectively. The percentage of pattern B sperm was measured by chlorotetracycline staining. Then the sperm were coincubated with 100 micromol/L W7 or 10 micromol/L calmidazolium (CZ) before acrosome reaction was induced by 5 micromol/L progesterone and evaluated by the same method. RESULTS: After the treatment of the sperm with different concentrations of CZ or W7, the percentages of pattern B sperm decreased in a dose-dependent manner, significantly different from the control (P < 0.05). There was a statistic difference in the rate of acrosome reaction between the experiment and the control group (P < 0.01). CONCLUSION: Calmodulin is a key protein involved in mouse sperm capacitation. FAU - Li, Kun AU - Li K AD - Department of Clinical Laboratory, Wenzhou Hospital of Integrated Traditional and Western Medicine, Wenzhou, Zhejiang 325000, China. FAU - Xue, Ya-Mei AU - Xue YM