• PMID: 18292846
• Gene Name: PRM1
• Condition: Asthenozoospermic
• Association: The findings indicate that a reduction in contents of PRM1 and PRM2 transcripts in spermatozoa may be linked with asthenozoospermia.
• Population size: 170
• Population details: 170 (70 normozoospermic, 100 asthenozoospermic)
• Sex: Male
• Infertility type: Male infertility
• Associated genes: PRM1, PRM2
• Other associated phenotypes: Asthenozoospermic

Evaluation of protamines 1 and 2 transcript contents in spermatozoa from asthenozoospermic men

Kempisty B, Depa-Martynow M, Lianeri M, Jedrzejczak P, Darul-Wasowicz A, Jagodzinski PP.

During mammalian spermatogenesis, the chromatin structure undergoes substantial condensation. The key role in this process is played by protamines 1 and 2 (PRM1, PRM2). We attempted to compare the levels of PRM1 and PRM2 transcripts in mature spermatozoa of normospermic and asthenozoospermic men. Human ejaculates from normozoospermic (n=70) and asthenozoospermic (n=100) donors were purified by centrifugation through discontinuous Percoll density gradient. RNA was isolated from spermatozoa according to the Chomczyñski and Sacchi method, treated with DNase I, and reverse-transcribed into cDNA. Using reverse transcription and real-time quantitative polymerase chain reaction analysis, we found a reduction in the levels of PRM1 and PRM2 transcripts in spermatozoa from asthenozoospermic men, as compared to controls (P<0.001). Our findings indicate that a reduction in contents of PRM1 and PRM2 transcripts in spermatozoa may be linked with asthenozoospermia. FAU - Kempisty, Bartosz AU - Kempisty B AD - Department of Biochemistry and Molecular Biology, University of Medical Sciences, Poznań, Poland. FAU - Depa-Martynow, Magdalena AU - Depa-Martynow M FAU - Lianeri, Margarita AU - Lianeri M FAU - Jedrzejczak, Piotr AU - Jedrzejczak P FAU - Darul-Wasowicz, Agnieszka AU - Darul-Wasowicz A