• PMID: 17334588
• Gene Name: CKLFSF2
• Condition: Spermatogenic defects
• Association: CKLFSF2 could play important roles in the process of meiosis and spermiogenesis, and might be involved in the vesicular transport or membrane apposition events in the endoplasmic reticulum.
• PMID: 17334588
• Gene Name: CKLFSF2
• Condition: Spermatogenic defects
• Association: CKLFSF2 could play important roles in the process of meiosis and spermiogenesis, and might be involved in the vesicular transport or membrane apposition events in the endoplasmic reticulum.
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: Spermatogenic defects

Expression and localization of CKLFSF2 in human spermatogenesis

Liu G, Xin ZC, Chen L, Tian L, Yuan YM, Song WD, Jiang XJ, Guo YL.

AIM: To investigate the expression and subcellular localization of chemokine-like factor superfamily 2 (CKLFSF2) in human testis and its potential role in spermatogenesis. METHODS: A specific polyclonal antibody against CKLFSF2 was raised. The expression and cellular localization of CKLFSF2 in the seminiferous tubules was checked by immunohistochemistry method. Also, in situ hybridization was applied to localize the mRNA distribution. The EGFP-CKLFSF2 fusion protein was expressed in COS-7 cells to localize its subcellular location in vitro. In addition, the abnormal expression of CKLFSF2 in testes of patients with male infertility was assayed by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry methods. RESULTS: Having a close correlation with spermatogenesis defects, CKLFSF2 was specifically expressed in meiotic and post-meiotic germ cells, which were localized to the endoplasmic reticulum (ER) near the Golgi apparatus. CONCLUSION: CKLFSF2 could play important roles in the process of meiosis and spermiogenesis, and might be involved in the vesicular transport or membrane apposition events in the endoplasmic reticulum. FAU - Liu, Gang AU - Liu G AD - Andrology Center, Peking University First Hospital, Beijing 100009, China. FAU - Xin, Zhong-Cheng AU - Xin ZC FAU - Chen, Liang AU - Chen L FAU - Tian, Long AU - Tian L FAU - Yuan, Yi-Ming AU - Yuan YM FAU - Song, Wei-Dong AU - Song WD FAU - Jiang, Xue-Jun AU - Jiang XJ