• PMID: 17133603
• Gene Name: DNM2
• Condition: May play a role in during acrosome formation and/or acrosomal exocytosis
• Association: Associated
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: May play a role in during acrosome formation and/or acrosomal exocytosis

Dynamin 2 associates with complexins and is found in the acrosomal region of mammalian sperm

Zhao L, Shi X, Li L, Miller DJ.

Previous data showed that complexin I, a SNARE regulatory protein, is localized in and/or around the acrosome and is necessary for the acrosome reaction in sperm. To understand how complexin I regulates the acrosome reaction, we used complexin-GST pulldown assays to identify interacting proteins. We showed that both complexins I and II bound mouse sperm dynamin 2. Dynamin 2 is a 100 kDa GTPase essential to many aspects of endocytosis but its potential role in exocytosis is unknown. Dynamin 2 is expressed in rat testis and widely expressed in other tissues; however, the function of dynamin 2 in germ cells is uncertain. Dynamin 2 protein was detected in mouse testis and was most abundant in or around the developing acrosome of spermatids. In addition, dynamin 2 was co-localized with complexin I in the acrosomal region of mammalian sperm. Its co-localization and interaction with complexin I suggest that dynamin 2 may play a role during acrosome formation and/or acrosomal exocytosis. CI - (c) 2006 Wiley-Liss, Inc. FAU - Zhao, Longmei AU - Zhao L AD - Department of Animal Sciences, University of Illinois, Urbana, Illinois 61801, USA. FAU - Shi, Xudong AU - Shi X FAU - Li, Lingjun AU - Li L