• PMID: 17007855
• Gene Name: HSPA2
• Condition: Varicocele
• Association: This is the first report on HSPA2 gene expression in ejaculated spermatozoa from adolescents and its relationship with varicocele pathology. Results demonstrated that HSPA2 expression was down-regulated in adolescents with varicocele and oligozoospermia c
• PMID: 17007855
• Gene Name: HSPA2
• Condition: Varicocele
• Association: Sperm from adolescents with varicocele and oligozoospermia had significantly lower levels of HSPA2 gene expression than both adolescents without varicocele (controls) and adolescents with varicocele and normal sperm concentration (P<.05). The latter group
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: Varicocele

Expression of the HSPA2 gene in ejaculated spermatozoa from adolescents with and without varicocele

Lima SB, Cenedeze MA, Bertolla RP, Filho PA, Oehninger S, Cedenho AP.

OBJECTIVE: To evaluate mRNA expression of the HSPA2 gene in ejaculated spermatozoa in adolescents with and without varicocele. DESIGN: Controlled prospective study. SETTING: Patients in an academic research environment. PATIENTS: Adolescent patients with clinical diagnosed bilateral varicocele grades II and III, and adolescent patients without varicocele. INTERVENTION: Reverse-transcription-polymerase chain reaction (RT-PCR) analysis of HSPA2 gene expression in adolescents with and without varicocele. MAIN OUTCOME MEASURE: Comparative RT-PCR expression analysis of HSPA2 gene mRNA, compared to the housekeeping beta-actin gene. RESULTS: Sperm from adolescents with varicocele and oligozoospermia had significantly lower levels of HSPA2 gene expression than both adolescents without varicocele (controls) and adolescents with varicocele and normal sperm concentration (P<.05). The latter group had a nonsignificant increase in gene expression compared to the control group. CONCLUSIONS: This is the first report on HSPA2 gene expression in ejaculated spermatozoa from adolescents and its relationship with varicocele pathology. Results demonstrated that HSPA2 expression was down-regulated in adolescents with varicocele and oligozoospermia compared to controls. There was a higher, albeit nonsignificant, gene expression in adolescents with varicocele and normal sperm concentration than in controls. We speculate that expression levels of this gene might be used as a molecular marker for the acquisition of thermal tolerance in ejaculated spermatozoa. FAU - Lima, Samira Barbosa AU - Lima SB AD - Division of Urology, Department of Surgery, São Paulo Federal University, São Paulo, Brazil. samirabl@uol.com.br FAU - Cenedeze, Marcos Antonio AU - Cenedeze MA FAU - Bertolla, Ricardo Pimenta AU - Bertolla RP FAU - Filho, Pericles Assad Hassun AU - Filho PA FAU - Oehninger, Sergio AU - Oehninger S