• PMID: 17001008
• Gene Name: PARP2
• Condition: Contributes to the fidelity of male meiosis I and spermiogenesis
• Association: Associated
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: Contributes to the fidelity of male meiosis I and spermiogenesis

Poly(ADP-ribose) polymerase-2 contributes to the fidelity of male meiosis I and spermiogenesis

Dantzer F, Mark M, Quenet D, Scherthan H, Huber A, Liebe B, Monaco L, Chicheportiche A, Sassone-Corsi P, de Murcia G, Ménissier-de Murcia J.

Besides the established central role of poly(ADP-ribose) polymerase-1 (Parp-1) and Parp-2 in the maintenance of genomic integrity, accumulating evidence indicates that poly(ADP-ribosyl)ation may modulate epigenetic modifications under physiological conditions. Here, we provide in vivo evidence for the pleiotropic involvement of Parp-2 in both meiotic and postmeiotic processes. We show that Parp-2-deficient mice exhibit severely impaired spermatogenesis, with a defect in prophase of meiosis I characterized by massive apoptosis at pachytene and metaphase I stages. Although Parp-2(-/-) spermatocytes exhibit normal telomere dynamics and normal chromosome synapsis, they display defective meiotic sex chromosome inactivation associated with derailed regulation of histone acetylation and methylation and up-regulated X- and Y-linked gene expression. Furthermore, a drastically reduced number of crossover-associated Mlh1 foci are associated with chromosome missegregation at metaphase I. Moreover, Parp-2(-/-) spermatids are severely compromised in differentiation and exhibit a marked delay in nuclear elongation. Altogether, our findings indicate that, in addition to its well known role in DNA repair, Parp-2 exerts essential functions during meiosis I and haploid gamete differentiation. FAU - Dantzer, Françoise AU - Dantzer F AD - Intégrité du Génome, Unité Mixte de Recherche 7175, Ecole Supérieure de Biotechnologie de Strasbourg, F-67412 Illkirch, France. f.dantzer@esbs.u-strasbg.fr FAU - Mark, Manuel AU - Mark M FAU - Quenet, Delphine AU - Quenet D FAU - Scherthan, Harry AU - Scherthan H FAU - Huber, Aline AU - Huber A FAU - Liebe, Bodo AU - Liebe B FAU - Monaco, Lucia AU - Monaco L FAU - Chicheportiche, Alexandra AU - Chicheportiche A FAU - Sassone-Corsi, Paolo AU - Sassone-Corsi P FAU - de Murcia, Gilbert AU - de Murcia G