• PMID: 16873132
• Gene Name: CYP19A1
• Condition: Male infertility
• Association: The decrease in CCR5 and P450arom transcripts in swim-up sperm may be associated with male infertility.
• Sex: Male
• Infertility type: Male infertility
• Associated genes: CCR5, P450arom
• Other associated phenotypes: Male infertility

Quantitative analysis of CCR5 chemokine receptor and cytochrome P450 aromatase transcripts in swim-up spermatozoa isolated from fertile and infertile men

Jedrzejczak P, Januchowski R, Taszarek-Hauke G, Laddach R, Pawelczyk L, Jagodzinski PP.

We determined the CCR5 chemokine receptor and cytochrome P450 aromatase (P450arom) transcript copies number in swim-up sperm isolated from fertile and infertile men. The ejaculates were purified by centrifugation through discontinuous Percoll density gradient and swim-up techniques. RNA was isolated from sperm, treated with DNase I and reverse-transcribed into cDNA. Quantitative analysis of CCR5 and P450arom cDNA were performed by real-time quantitative (RQ-PCR) SYBR Green I analysis. There was a higher content of CCR5 and P450arom transcripts copy number in swim-up sperm of fertile than from infertile donors. The decrease in CCR5 and P450arom transcripts in swim-up sperm may be associated with male infertility. FAU - Jedrzejczak, P AU - Jedrzejczak P AD - Department of Infertility and Reproductive Endocrinology, Poznań University of Medical Sciences, Poznań, Poland. FAU - Januchowski, R AU - Januchowski R FAU - Taszarek-Hauke, G AU - Taszarek-Hauke G FAU - Laddach, R AU - Laddach R FAU - Pawelczyk, L AU - Pawelczyk L