About Us |
PMID | 16804880 |
Gene Name | DRC7 |
Condition | May play definite roles regulating differentiation of germ cells during spermatogenesis, particularly during meiosis and spermiogenesis |
Association |
Associated |
Sex | Male |
Infertility type | Male infertility |
Other associated phenotypes |
May play definite roles regulating differentiation of germ cells during spermatogenesis, particularly during meiosis and spermiogenesis |
Molecular cloning and characterization of SRG-L, a novel mouse gene developmentally expressed in spermatogenic cells Ma Q, Wang H, Guo R, Wang H, Ge Y, Ma J, Xue S, Han D. Full-length cDNA of a novel mouse gene upregulated in late stages of spermatogenic cells was cloned from mouse testis using overlapping RT-PCR and RACE. The mRNA of the gene was expressed mainly in diplotene/pachytene spermatocytes, round and elongating spermatids. We named this gene as SRG-L (Spermatogenesis Related Gene expressed in late stages of spermatogenic cells, GenBank Accession No. AY352586). The tissue-specific analysis showed a higher expression level in testis and spleen. The gene is mapped on chromosome 8q33.1 and contains 18 exons. The full-length of cDNA is 2,843 bp with an open reading frame (ORF) of 2,625 bp that encodes a 104 kDa protein (874 amino acids) with a putative transmembrane region. The bioinformatics analysis revealed that the SRG-L has two conserved regions, transglutaminase-like homologues domain and D-serine dehydratase domain, rich phosphorylation sites and methylation sites. The SRG-L protein was detected in diplotene/pachytene spermatocytes and spermatids by immunohistochemical staining and Western blot. The results suggest that SRG-L may play definite roles regulating differentiation of germ cells during spermatogenesis, particularly during meiosis and spermiogenesis. FAU - Ma, Quanhong AU - Ma Q AD - Department of Cell Biology, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing, China. FAU - Wang, Haikun AU - Wang H FAU - Guo, Rui AU - Guo R FAU - Wang, Huizhen AU - Wang H FAU - Ge, Yehua AU - Ge Y FAU - Ma, Jing AU - Ma J FAU - Xue, Shepu AU - Xue S |