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PMID 16804880
Gene Name DRC7
Condition May play definite roles regulating differentiation of germ cells during spermatogenesis, particularly during meiosis and spermiogenesis
Association Associated
Sex Male
Infertility type Male infertility
Other associated phenotypes May play definite roles regulating differentiation of germ cells during spermatogenesis, particularly during meiosis and spermiogenesis


Molecular cloning and characterization of SRG-L, a novel mouse gene developmentally expressed in spermatogenic cells

Ma Q, Wang H, Guo R, Wang H, Ge Y, Ma J, Xue S, Han D.

Full-length cDNA of a novel mouse gene upregulated in late stages of spermatogenic cells was cloned from mouse testis using overlapping RT-PCR and RACE. The mRNA of the gene was expressed mainly in diplotene/pachytene spermatocytes, round and elongating spermatids. We named this gene as SRG-L (Spermatogenesis Related Gene expressed in late stages of spermatogenic cells, GenBank Accession No. AY352586). The tissue-specific analysis showed a higher expression level in testis and spleen. The gene is mapped on chromosome 8q33.1 and contains 18 exons. The full-length of cDNA is 2,843 bp with an open reading frame (ORF) of 2,625 bp that encodes a 104 kDa protein (874 amino acids) with a putative transmembrane region. The bioinformatics analysis revealed that the SRG-L has two conserved regions, transglutaminase-like homologues domain and D-serine dehydratase domain, rich phosphorylation sites and methylation sites. The SRG-L protein was detected in diplotene/pachytene spermatocytes and spermatids by immunohistochemical staining and Western blot. The results suggest that SRG-L may play definite roles regulating differentiation of germ cells during spermatogenesis, particularly during meiosis and spermiogenesis. FAU - Ma, Quanhong AU - Ma Q AD - Department of Cell Biology, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing, China. FAU - Wang, Haikun AU - Wang H FAU - Guo, Rui AU - Guo R FAU - Wang, Huizhen AU - Wang H FAU - Ge, Yehua AU - Ge Y FAU - Ma, Jing AU - Ma J FAU - Xue, Shepu AU - Xue S