• PMID: 16720623
• Gene Name: CDC25A
• Condition: Azoospermia, spermatogenic failure, male infertility
• Association: Decreased CDC25A is associated with spermatogenic failure and failed sperm retrieval in infertile men. Further studies are necessary to explore the functional roles of CDC25A in human spermatozoa.
• Population size: 40
• Population details: 40 azoospermic men
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: Azoospermia, spermatogenic failure, male infertility

Association of spermatogenic failure with decreased CDC25A expression in infertile men

Cheng YS, Kuo PL, Teng YN, Kuo TY, Chung CL, Lin YH, Liao RW, Lin JS, Lin YM.

BACKGROUND: DAZ gene family is crucial for human spermatogenesis that requires the precise co-ordination of cell cycle events. CDC25A is recognized as the downstream substrate of DAZ gene family and is thought to function on the M-phase regulation of cell cycles. We investigated the expression profiles of CDC25A in the testes of infertile men and evaluated the relationship between CDC25A levels and testicular phenotype, clinical hormonal parameters and sperm retrieval results. METHODS: The protein and mRNA transcript levels of CDC25A in the testes of 40 azoospermic men were determined by immunohistochemistry and quantitative real-time-PCR. CDC25A in human spermatozoa was investigated by western blotting and immunofluorescence staining. RESULTS: The CDC25A protein was expressed mainly in spermatocyte, spermatid and spermatozoa. CDC25A transcript levels were significantly decreased (P = 0.0009) in patients with spermatogenic failure, especially in men with meiotic arrest and Sertoli cell-only syndrome. Significantly higher CDC25A transcript levels were detected in patients with successful sperm retrieval than in patients with failed sperm retrieval (P = 0.005). CONCLUSIONS: Decreased CDC25A is associated with spermatogenic failure and failed sperm retrieval in infertile men. Further studies are necessary to explore the functional roles of CDC25A in human spermatozoa. FAU - Cheng, Yu Sheng AU - Cheng YS AD - Department of Urology, National Cheng Kung University, Tainan, Taiwan. FAU - Kuo, Pao Lin AU - Kuo PL FAU - Teng, Yen Ni AU - Teng YN FAU - Kuo, Ting Yi AU - Kuo TY FAU - Chung, Chia Ling AU - Chung CL FAU - Lin, Ying Hung AU - Lin YH FAU - Liao, Rui Wen AU - Liao RW FAU - Lin, Johnny Shinn Nan AU - Lin JS