• PMID: 1652567
• Gene Name: TP53
• Condition: Testicular cancer
• Association: Altered expression of the p53 protein is therefore a unifying feature of the majority of invasive male germ-cell tumours and the change resulting in high levels of p53 appears to be a relatively early step in the human testicular cancer pathogenesis.
• Population size: 107
• Population details: 107 cases of testicular cancer
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: Testicular cancer

p53 protein alterations in human testicular cancer including pre-invasive intratubular germ-cell neoplasia

Bártková J, Bártek J, Lukás J, Vojt?sek B, Stasková Z, Rejthar A, Kovarík J, Midgley CA, Lane DP.

Expression of the p53 oncoprotein was examined in a wide range of primary human testicular germ-cell tumours using a new mouse monoclonal antibody (MAb) BP53-11 raised and characterized in this study, in parallel with a polyclonal rabbit antiserum CM-1. Immunohistochemistry on paraffin sections showed positive nuclear reaction in at least a fraction of malignant cells in 90 (84%) out of 107 cases studied. Aberrant accumulation of the p53 protein was found among testicular tumours of all major histological types, although generally a higher percentage of positive cases and a higher proportion of p53 over-expressing nuclei within individual lesions was observed in embryonal carcinomas when compared with seminomas. The typical heterogeneous staining pattern characteristic of histological specimens was also found in a cultured cell line derived from a human embryonal carcinoma. In contrast to immunohistochemically undetectable levels in normal testes and morphologically normal tissue areas in the tumour-bearing testes, the accumulation of the p53 protein was clearly identified in a high proportion (59% of cases) of the pre-invasive lesions with positive atypical intratubular germ cells often found in the tissue adjacent to invasive tumours. Altered expression of the p53 protein is therefore a unifying feature of the majority of invasive male germ-cell tumours and the change resulting in high levels of p53 appears to be a relatively early step in the human testicular cancer pathogenesis. FAU - Bártková, J AU - Bártková J AD - Masaryk Institute of Oncology, Brno, Czechoslovakia. FAU - Bártek, J AU - Bártek J FAU - Lukás, J AU - Lukás J FAU - Vojtĕsek, B AU - Vojtĕsek B FAU - Stasková, Z AU - Stasková Z FAU - Rejthar, A AU - Rejthar A FAU - Kovarík, J AU - Kovarík J FAU - Midgley, C A AU - Midgley CA