• PMID: 155335
• Gene Name: INSL3
• Condition: Infertility
• Association: The study identified polymorphic alleles of INSL3 and GREAT, but no deleterious mutations among individuals with familial cryptorchidism which implies that mutations in these two genes are responsible only for a small proportion of familial cryptorchidis
• Mutation: 2 silent substitutions and one missense (A60T) substitution in exon 1
• Population size: 13
• Population details: 13 patients with cryptorchidism
• Sex: Male
• Infertility type: Male infertility
• Associated genes: Great/Lgr8
• Other associated phenotypes: Cryptorchidism

Stimulation of allogeneic lymphocytes by skin epidermal cells in the rat

Tanaka S, Sakai A.

The ability of skin epidermal cells to induce allogeneic lymphocytes into proliferation was examined in mixed skin cell-lymphocyte culture reaction (MSLR). The stimulating capacity of skin cells was reduced significantly by trypsin digestion, although the damage was repaired by incubation at 37 C for 3 hr. The optimal concentration of mitomycin C for treatment of stimulating cells in the MSLR differed from that in mixed lymphocyte culture reaction (MLR). Irradiation rendered them three to four times more stimulatory than did mitomycin C. Removal of adherent cells from responding cells by passage through a nylon-wool column gave a substantial elevation of the MSLR. The lymphocytes cocultured with skin cells in the primary MSLR incorporated 3H-thymidine, with the peak at the 6th day of culture. If the lymphocytes primed in the MSLR were restimulated with skin cells from the same stimulating strain, the primed lymphocytes responded promptly and in great magnitude. FAU - Tanaka, S AU - Tanaka S