• PMID: 15533496
• Gene Name: IL-6
• Condition: Varicocele, male infertility
• Association: Associated
• Population size: 50
• Population details: 50 (15 fertile donors (controls), 35 infertile patients with varicocele)
• Sex: Male
• Infertility type: Male infertility
• Other associated phenotypes: Varicocele, male infertility

Relationship of interleukin-6 with semen characteristics and oxidative stress in patients with varicocele

Nallella KP, Allamaneni SS, Pasqualotto FF, Sharma RK, Thomas AJ Jr, Agarwal A.

OBJECTIVES: To examine levels of interleukin-6 (IL-6) in fertile semen donors and patients with varicocele and examine its association with semen characteristics and levels of reactive oxygen species (ROS). METHODS: We conducted a prospective study consisting of 15 fertile donors (controls) and 35 infertile patients with varicocele. Semen analysis was performed according to the World Health Organization guidelines. IL-6 levels were measured using the enzyme-linked immunosorbent assay. ROS (x10(4) counted photons per minute per 20 x 10(6) sperm) and total antioxidant capacity (molar trolox equivalents) were measured using a chemiluminescence assay. RESULTS: The sperm concentration and motility were significantly greater in the donors compared with the infertile patients with varicocele (P <0.0001 and P = 0.01, respectively). The IL-6 (log10 [IL-6 +1]) and ROS (log10 [ROS +1]) levels were significantly greater in infertile patients with varicocele than in the donors (IL-6: 2.1 [1.7, 2.4] versus 0.7 [0, 1.9], P = 0.003; ROS: 1.8 [1.2, 2.6] versus 1.0 [0.7, 1.6], P = 0.04). The total antioxidant capacity levels were significantly lower in the varicocele patients (1166.7 +/- 366.2) than in the donors (1556.4 +/- 468.1; P = 0.003). The IL-6 levels correlated significantly with the ROS levels in the infertile patients with varicocele (r = -0.39; P = 0.01). CONCLUSIONS: Infertile patients with varicocele exhibited elevated levels of IL-6 and ROS and decreased levels of total antioxidant capacity. Pro-inflammatory cytokine IL-6 and oxidative stress may play a role in the pathophysiology of infertility in these patients. FAU - Nallella, Kiran P AU - Nallella KP AD - Center for Advanced Research in Human Reproduction, Infertility and Sexual Function, Glickman Urological Institute and Department of Obstetrics and Gynecology, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA. FAU - Allamaneni, Shyam S R AU - Allamaneni SS FAU - Pasqualotto, Fabio F AU - Pasqualotto FF FAU - Sharma, Rakesh K AU - Sharma RK FAU - Thomas, Anthony J Jr AU - Thomas AJ Jr